Lai Jengyu, Pittelkow Mark R
Department of Dermatology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA.
Int J Dermatol. 2007 Jun;46(6):587-93. doi: 10.1111/j.1365-4632.2007.02914.x.
Chronic wounds present an increasing challenge in healthcare and consume a substantial portion of healthcare cost. Although new treatments have been developed, treatment success has not been improved greatly. Ultrasound has long been employed in medicine. Its unique ability to deliver energy makes it an ideal candidate as a wound care modality. We proposed that ultrasound would differentially affect intracellular signaling pathways and, with the ability to assess this effect using a noncontact form of ultrasound, were provided with a means to test this proposal.
The cellular morphology, mitogenic activities, expression of keratinocyte growth factor (KGF) and transforming growth factor beta-1 (TGF-beta1), and activation of extracellular regulated kinase (ERK) and c-Jun N-terminal kinase (JNK) signaling pathways of dermal fibroblasts were studied after ultrasound treatment. Untreated and scrape-wounded fibroblasts were utilized as controls.
There was no difference in morphology observed, except for vacuolization in ultrasound-treated fibroblasts. Mitogenic activities were similar between ultrasound-treated and scrape-wounded fibroblasts. Ultrasound-treated fibroblasts exhibited a much earlier increase in KGF expression, ERK activation, and JNK activation. The ERK/JNK ratio was increased markedly in ultrasound-treated fibroblasts.
We conclude that ultrasound induces cellular responses that may be beneficial to wound healing.
慢性伤口给医疗保健带来了日益严峻的挑战，并消耗了很大一部分医疗费用。尽管已经开发出了新的治疗方法，但治疗成功率并未得到显著提高。超声长期以来一直在医学中得到应用。其传递能量的独特能力使其成为伤口护理方式的理想选择。我们提出超声会对细胞内信号通路产生不同影响，并且由于能够使用非接触形式的超声评估这种影响，从而为测试这一假设提供了一种方法。
研究了超声处理后真皮成纤维细胞的细胞形态、促有丝分裂活性、角质形成细胞生长因子（KGF）和转化生长因子β1（TGF-β1）的表达，以及细胞外调节激酶（ERK）和c-Jun氨基末端激酶（JNK）信号通路的激活情况。未处理和刮伤的成纤维细胞用作对照。
除了超声处理的成纤维细胞出现空泡化外，未观察到形态上的差异。超声处理的成纤维细胞和刮伤的成纤维细胞之间的促有丝分裂活性相似。超声处理的成纤维细胞中KGF表达、ERK激活和JNK激活的增加要早得多。超声处理的成纤维细胞中ERK/JNK比值显著增加。
我们得出结论，超声诱导的细胞反应可能有利于伤口愈合。
