Bohari Siti Pm, Grover Liam M, Hukins David Wl
Faculty of Biosciences and Biomedical Engineering, Universiti Teknologi Malaysia, Johor Bahru, Malaysia ; School of Chemical Engineering, University of Birmingham, Birmingham, UK ; School of Mechanical Engineering, University of Birmingham, Birmingham, UK.
School of Chemical Engineering, University of Birmingham, Birmingham, UK.
J Tissue Eng. 2015 Nov 19;6:2041731415615777. doi: 10.1177/2041731415615777. eCollection 2015 Jan-Dec.
This study evaluated the effect of pulsed low-intensity ultrasound on cell proliferation, collagen production and glycosaminoglycan deposition by human dermal fibroblasts encapsulated in alginate. Hoechst 33258 assay for cell number, hydroxyproline assay for collagen content, dimethylmethylene blue assay for glycosaminoglycan content and scanning electron microscopy were performed on the encapsulated cells treated with pulsed low-intensity ultrasound and a control group that remained untreated. Pulsed low-intensity ultrasound showed a significant effect on cell proliferation and collagen deposition but no consistent pattern for glycosaminoglycan content. Alcian blue staining showed that glycosaminoglycans were deposited around the cells in both treated and control groups. These results suggest that pulsed low-intensity ultrasound alone shows a positive effect on cell proliferation and collagen deposition even without growth factor supplements.
本研究评估了脉冲低强度超声对封装在藻酸盐中的人皮肤成纤维细胞的细胞增殖、胶原蛋白生成和糖胺聚糖沉积的影响。对经脉冲低强度超声处理的封装细胞和未处理的对照组进行了用于细胞计数的Hoechst 33258检测、用于胶原蛋白含量的羟脯氨酸检测、用于糖胺聚糖含量的二甲基亚甲基蓝检测以及扫描电子显微镜检查。脉冲低强度超声对细胞增殖和胶原蛋白沉积有显著影响，但糖胺聚糖含量没有一致的模式。阿尔辛蓝染色显示，处理组和对照组的细胞周围均有糖胺聚糖沉积。这些结果表明，即使不添加生长因子，单独的脉冲低强度超声对细胞增殖和胶原蛋白沉积也有积极作用。
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