[考纳斯及其辖区终末期肾衰竭血液透析患者中汉坦病毒抗体的流行情况]
[Prevalence of antibodies to hantavirus among hemodialysis patients with end-stage renal failure in Kaunas and its district].
作者信息
Dargevicius Arvydas, Petraityte Rasa, Sribikiene Birute, Sileikiene Elvyra, Razukeviciene Loreta, Ziginskiene Edita, Vorobjoviene Rita, Razanskiene Ausra, Sasnauskas Kestutis, Bumblyte Inga Arūne, Kuzminskis Vytautas
机构信息
Department of Nephrology, Kaunas University of Medicine, Kaunas, Lithuania.
出版信息
Medicina (Kaunas). 2007;43 Suppl 1:72-6.
UNLABELLED
The objective of this study was to investigate the prevalence of antibodies to hantaviruses among hemodialysis patients with end-stage renal failure in Kaunas and its district.
MATERIAL AND METHODS
Serums of 218 patients from four dialysis centers of Kaunas district were tested by using the immunoglobulin G antibody-capture enzyme-linked immunosorbent assay (ELISA). The reactivity of ELISA-positive sera was proven in Western blot tests using various hantavirus recombinant nucleocapsid proteins. The yeast-expressed nucleocapsid proteins were used for testing.
RESULTS
Antibodies against Dobrava/Hantaan and Puumala hantaviruses were found in 16 patients (seroprevalence 7.4%). Most of the sera were positive for Dobrava hantavirus (81%). The ratio of males to females was 1.2:1. Seroprevalence was significantly higher in older patients.
CONCLUSIONS
Results indicate that antibodies to two hantaviruses (Dobrava/Hantaan virus and Puumala virus) are prevalent among hemodialysis patients in Kaunas district with approximately the same seroprevalence as in neighboring countries.
未标注
本研究的目的是调查考纳斯及其辖区内终末期肾衰竭血液透析患者中汉坦病毒抗体的流行情况。
材料与方法
采用免疫球蛋白G抗体捕获酶联免疫吸附测定法（ELISA）检测考纳斯地区四个透析中心218例患者的血清。使用各种汉坦病毒重组核衣壳蛋白通过蛋白质印迹试验证实ELISA阳性血清的反应性。使用酵母表达的核衣壳蛋白进行检测。
结果
在16例患者中发现了针对多布拉瓦/汉滩病毒和普马拉汉坦病毒的抗体（血清阳性率为7.4%）。大多数血清对多布拉瓦汉坦病毒呈阳性（81%）。男女比例为1.2:1。老年患者的血清阳性率明显更高。
结论
结果表明，考纳斯地区血液透析患者中两种汉坦病毒（多布拉瓦/汉滩病毒和普马拉病毒）的抗体流行，血清阳性率与邻国大致相同。
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