紫外线C照射的枯草芽孢杆菌的DNA双嘧啶光产物修复及转录反应

DNA bipyrimidine photoproduct repair and transcriptional response of UV-C irradiated Bacillus subtilis.

作者信息

Moeller Ralf, Stackebrandt Erko, Douki Thierry, Cadet Jean, Rettberg Petra, Mollenkopf Hans-Joachim, Reitz Günther, Horneck Gerda

机构信息

German Aerospace Center (DLR), Radiation Biology Division, Institute of Aerospace Medicine, Linder Hoehe, 51147 Cologne, Germany.

出版信息

Arch Microbiol. 2007 Oct;188(4):421-31. doi: 10.1007/s00203-007-0263-4. Epub 2007 Jun 6.

DOI:10.1007/s00203-007-0263-4

PMID:17551712

Abstract

Vegetative wild-type and DNA repair-deficient (homologous recombination, recA and nucleotide excision repair, uvrB) Bacillus subtilis cells were exposed to UV-C radiation. Colony formation, DNA bipyrimidine photoproducts and gene expression were measured during cell recovery. Gene expression was measured after 60 min cell recovery where 50% (wild-type), 30% (recA) and 8% (uvrB), respectively, of the UV-C induced DNA photoproducts were repaired. We examined changes in the gene expression following UV exposure in wild-type and both repair-deficient strains. A set of known and unknown genes were found to be significantly up-regulated in wild-type B. subtilis cells, whereas no or lower gene induction was determined for both mutant strains. In addition, the possible roles of newly identified UV-responsive genes are discussed with respect to cellular recovery following exposure to UV irradiation.

摘要

将野生型和DNA修复缺陷型（同源重组，recA；核苷酸切除修复，uvrB）的枯草芽孢杆菌细胞暴露于UV-C辐射下。在细胞恢复过程中测量菌落形成、DNA双嘧啶光产物和基因表达。在细胞恢复60分钟后测量基因表达，此时UV-C诱导的DNA光产物分别有50%（野生型）、30%（recA）和8%（uvrB）得到修复。我们研究了野生型和两种修复缺陷型菌株在紫外线照射后基因表达的变化。发现一组已知和未知基因在野生型枯草芽孢杆菌细胞中显著上调，而两种突变菌株均未检测到基因诱导或基因诱导较低。此外，还讨论了新鉴定的紫外线响应基因在紫外线照射后细胞恢复方面的可能作用。