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[高效液相色谱法同时测定灵芝中四种三萜酸]

[Simultaneous HPLC determination of four triterpenoid acids in Ganoderma lucidum].

作者信息

Zhao Jia, Chen Xiaohui, Bi Kaishun

机构信息

School of Traditional Chinese Materia Madica, Shenyang Pharmaceutical University, Shenyang 110016, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2009 Sep;34(17):2220-2.

PMID:19943490

Abstract

OBJECTIVE

To determine 4 kinds of triterpenoid acids in Ganodermna lucidum, namely ganoderic acid C2, ganoderenic acid A, ganoderic acid A and ganoderic acid D quantitively.

METHOD

The RP-HPLC method was applied and the separation was performed on a Kromasil C18 analytical column (4.6 mm x 250 mm, 5 microm). The mobile phase was acetonitrile (A)-water containing 0.03% H3PO4 (B) with gradient elution mode at the flow rate of 1.0 mL x min(-1). The detection was set at 252 nm, and the column temperature was 35 degrees C.

RESULT

The linear ranges of ganoderic acid C2, ganoderenic acid A, ganoderic acid A and ganoderic acid D were 5.0-50.0 microg x mL(-1) (r = 0.9999), 7.2-72 mg x L(-1) (r = 0.9998), 11.67-116.7 mg x L(-1) (r = 0.9999), 5.32-53.2 mg x L(-1) (r = 0.9996), respectively. The average recoveries (n=9) were 98.8% (RSD 1.5%), 99.1% (RSD 1.9%), 99.5% (RSD 1.4%), 98.5% (RSD 1.9%), respectively.

CONCLUSION

The method is simple and accurate with a good reproducibility and can be used as a quality control method for G. lucidum of different sources.

摘要

目的

定量测定灵芝中4种三萜酸，即灵芝酸C2、灵芝烯酸A、灵芝酸A和灵芝酸D。

方法

采用反相高效液相色谱法，在Kromasil C18分析柱（4.6 mm×250 mm，5μm）上进行分离。流动相为乙腈（A）-含0.03% H3PO4的水（B），采用梯度洗脱模式，流速为1.0 mL·min-1。检测波长设定为252 nm，柱温为35℃。

结果

灵芝酸C2、灵芝烯酸A、灵芝酸A和灵芝酸D的线性范围分别为5.0 - 50.0μg·mL-1（r = 0.9999）、7.2 - 72 mg·L-1（r = 0.9998）、11.67 - 116.7 mg·L-1（r = 0.9999）、5.32 - 53.2 mg·L-1（r = 0.9996）。平均回收率（n = 9）分别为98.8%（RSD 1.5%）、99.1%（RSD 1.9%）、99.5%（RSD 1.4%）、98.5%（RSD 1.9%）。