[垂盆草中三萜类化合物δ-香树脂酮的测定]
[Determination of triterpene delta-amyrone in Sedum sarmentosum].
作者信息
Cao Su-ping, Liu Li-fang, Wang Qiang
机构信息
Department of Chinese Pharmceutical Analysis, China Pharmaceutical University, Nanjing.
出版信息
Zhongguo Zhong Yao Za Zhi. 2006 Jan;31(1):41-2, 50.
OBJECTIVE
To establish the quantitative analysis method of triterpene delta-amyrone in Sedum sarmentosum by HPLC-ELSD.
METHOD
The Zorbax Sil Column was used and the mobile phase was dichloromethane-methanol (99:1). The flow rate was set at 0.5 mL x min(-1), the temperature of drift tube was set at 100 degrees C and the nebulizer nitrogen flow rate was 3.0 L x min(-1).
RESULT
The linear range of sigma-amyrone was 2.12-10.6 microg. The average recovery was 100.5%.
CONCLUSION
The results showed that the method was reliable, simple and precise. It would be beneficial for the study of the quality control of S. sarmentosum.
目的
建立用高效液相色谱-蒸发光散射检测器（HPLC-ELSD）测定垂盆草中三萜类成分δ-香树脂酮含量的定量分析方法。
方法
采用Zorbax Sil柱，以二氯甲烷-甲醇（99:1）为流动相，流速为0.5 mL·min⁻¹，漂移管温度设定为100℃，雾化器氮气流量为3.0 L·min⁻¹。
结果
δ-香树脂酮的线性范围为2.12 - 10.6 μg，平均回收率为100.5%。
结论
结果表明该方法可靠、简便、准确，有助于垂盆草质量控制的研究。
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