Wolfe Lisa L, Spraker Terry R, González Lorenzo, Dagleish Mark P, Sirochman Tracey M, Brown Jeremy C, Jeffrey Martin, Miller Michael W
Colorado Division of Wildlife, Wildlife Research Center, 317 West Prospect Road, Fort Collins, CO 80526-2097, USA.
Colorado State University Veterinary Diagnostic Laboratory, Colorado State University, Fort Collins, CO 80523, USA.
J Gen Virol. 2007 Jul;88(Pt 7):2078-2082. doi: 10.1099/vir.0.82342-0.
The utility of rectal lymphoid tissue sampling for the diagnosis of chronic wasting disease (CWD) infections in mule deer (Odocoileus hemionus) and white-tailed deer (Odocoileus virginianus) was evaluated. CWD-associated prion protein (PrP(CWD)) deposits were observed in the rectal mucosa from 19 orally inoculated mule deer by 381 days post-inoculation (p.i.); similarly, 45 out of 50 naturally infected mule deer had PrP(CWD) in their rectal mucosa. In orally inoculated white-tailed deer, the presence of glycine (G) or serine (S) at codon 96 of the native PrP (denoted 96GG, 96GS or 96SS) appeared to influence the temporal patterns of PrP(CWD) deposition: nine out of 11 infected 96GG individuals had PrP(CWD) in their rectal mucosa by 342 days p.i., whereas only three out of seven infected 96GS individuals had PrP(CWD) in their rectal mucosa by 381 days p.i. and none of three 96SS individuals had PrP(CWD) in their rectal mucosa by 751 days p.i. These findings support further evaluation of rectal mucosa sampling in CWD surveillance.
评估了直肠淋巴组织采样在诊断骡鹿（白尾鹿属）和白尾鹿（弗吉尼亚白尾鹿）慢性消耗病（CWD）感染中的效用。在接种后381天，从19只经口接种的骡鹿的直肠黏膜中观察到了与CWD相关的朊病毒蛋白（PrP(CWD)）沉积；同样，50只自然感染的骡鹿中有45只在其直肠黏膜中检测到PrP(CWD)。在经口接种的白尾鹿中，天然PrP第96密码子处甘氨酸（G）或丝氨酸（S）的存在（分别表示为96GG、96GS或96SS）似乎影响PrP(CWD)沉积的时间模式：11只感染的96GG个体中有9只在接种后342天其直肠黏膜中检测到PrP(CWD)，而7只感染的96GS个体中只有3只在接种后381天其直肠黏膜中检测到PrP(CWD)，3只96SS个体在接种后751天其直肠黏膜中均未检测到PrP(CWD)。这些发现支持在CWD监测中进一步评估直肠黏膜采样。
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