Elzanaty Saad, Malm Johan
Reproductive Medicine Center, Scanian Andrology Center, Lund University, Malmö University Hospital, Malmö, Sweden.
J Androl. 2007 Nov-Dec;28(6):847-52. doi: 10.2164/jandrol.107.002659. Epub 2007 Jun 6.
This study aimed to examine the association between the interval from ejaculation to analysis and epididymal and accessory sex gland function in relation to sperm motility. Ejaculates from 1079 men assessed for infertility were analyzed according to World Health Organization guidelines. Biochemical markers were measured in semen to assess the function of the epididymis (neutral alpha-glucosidase [NAG]), prostate (prostate-specific antigen [PSA] and zinc), and seminal vesicles (fructose). Three groups were defined according to time from ejaculation to analysis: G(< or =30) (24-30 minutes), G(31-60) (31-60 minutes), and G(>60) (63-180 minutes). The proportion of progressively motile sperm was significantly lower in G(>60) than in G(< or =30) (mean difference, 8.0%; 95% confidence interval [CI], 2.0%-13%) or G(31-60) (mean difference, 6.0%; 95% CI, 1.0%-12%). The proportion of rapid progressive sperm motility was significantly higher in G(< or =30) compared with G(31-60) (mean difference, 3.0%; 95% CI, 1.0%-5.0%) and G(>60) (mean difference, 6.0%; 95% CI, 1.0%-10%). Sperm morphology and viability did not vary significantly between the groups. However, PSA levels in G(>60) were 29% and 31% significantly lower than in G(< or =30) (95% CI, 3.0%-54%) and G(31-60) (95% CI, 7.0%-58%), respectively. Moreover, men in G(>60) had 29% and 17% significantly lower zinc compared with those in G(< or =30) (95% CI, 4.0%-69%) and G(31-60) (95% CI, 4.0%-64%), respectively. Levels of NAG and fructose did not differ significantly between the groups. There were negative associations between the ejaculation-to-analysis interval and sperm motility and levels of PSA and zinc. In male infertility assessments, semen analysis should be performed within 60 minutes of ejaculation.
本研究旨在探讨射精至分析的时间间隔与附睾及附属性腺功能之间的关联，以及其与精子活力的关系。根据世界卫生组织指南，对1079名因不育接受评估的男性的射精样本进行了分析。检测精液中的生化标志物，以评估附睾（中性α-葡萄糖苷酶 [NAG]）、前列腺（前列腺特异性抗原 [PSA] 和锌）以及精囊（果糖）的功能。根据射精至分析的时间定义了三组：G(≤30)（24 - 30分钟）、G(31 - 60)（31 - 60分钟）和G(>60)（63 - 180分钟）。G(>60)组中进行性运动精子的比例显著低于G(≤30)组（平均差异为8.0%；95%置信区间 [CI]，2.0% - 13%）或G(31 - 60)组（平均差异为6.0%；95% CI，1.0% - 12%）。与G(31 - 60)组（平均差异为3.0%；95% CI，1.0% - 5.0%）和G(>60)组（平均差异为6.0%；95% CI，1.0% - 10%）相比，G(≤30)组中快速进行性运动精子的比例显著更高。各组之间精子形态和活力无显著差异。然而，G(>60)组中的PSA水平分别比G(≤30)组（95% CI，3.0% - 54%）和G(31 - 60)组（95% CI，7.0% - 58%）显著低29%和31%。此外，与G(≤30)组（95% CI，4.0% - 69%）和G(31 - 60)组（95% CI，4.0% - 64%）相比，G(>60)组男性的锌含量分别显著低29%和17%。各组之间NAG和果糖水平无显著差异。射精至分析的时间间隔与精子活力以及PSA和锌水平之间存在负相关。在男性不育评估中，精液分析应在射精后60分钟内进行。
