嗜酸氧化亚铁硫杆菌KKS102来源的 Rieske型[2Fe-2S]铁氧化还原蛋白与NADH依赖型铁氧化还原蛋白还原酶电子传递复合物的结晶及初步X射线分析
Crystallization and preliminary X-ray analysis of the electron-transfer complex of Rieske-type [2Fe-2S] ferredoxin and NADH-dependent ferredoxin reductase derived from Acidovorax sp. strain KKS102.
作者信息
Senda Miki, Kishigami Shinya, Kimura Shigenobu, Senda Toshiya
机构信息
Japan Biological Information Research Center, Japan Biological Infomatics Consortium, Aomi, Koto-ku, Tokyo, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jun 1;63(Pt 6):520-3. doi: 10.1107/S1744309107023007. Epub 2007 May 18.
Abstract
The electron-transfer complex of BphA3, a Rieske-type [2Fe-2S] ferredoxin, and BphA4, a NADH-dependent ferredoxin reductase, was crystallized using the sitting-drop vapour-diffusion method under anaerobic conditions. The obtained crystals were analyzed by SDS-PAGE, which showed that they contained both BphA3 and BphA4. The crystals belong to space group P2(1), with unit-cell parameters a = 60.60, b = 173.72, c = 60.98 A, beta = 115.8 degrees, and diffracted to a resolution of 1.9 A.
摘要
在厌氧条件下，使用坐滴气相扩散法使BphA3（一种Rieske型[2Fe-2S]铁氧化还原蛋白）与BphA4（一种依赖NADH的铁氧化还原蛋白还原酶）的电子转移复合物结晶。通过SDS-PAGE对获得的晶体进行分析，结果表明晶体中同时含有BphA3和BphA4。这些晶体属于空间群P2(1)，晶胞参数为a = 60.60、b = 173.72、c = 60.98 Å，β = 115.8°，衍射分辨率为1.9 Å。
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