Xia Shi-Tou, Xiao Lang-Tao, Bi Dong-Lin, Zhu Zhao-Hai
Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha 410128, China.
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2007 Jun;33(3):179-87.
Replication factor C (RFC), consisting of one large subunit and four small subunits, is an important factor involved in DNA replication and repair mechanisms as well as cell proliferation. The subunit 1 of Arabidopsis RFC (AtRFC1) is a homologue of p140, the large subunit of human RFC. Three T-DNA insertion mutant lines of AtRFC1, i.e. rfc1-1, rfc1-2 and rfc1-3, with insertion mutations located in exons 16 and 19, and the promoter region respectively were verified. These mutations caused defects in embryogenesis and led to embryo and seed abortion. Transformation of wild type AtRFC1 gene into rfc1 mutant alleles reverted the mutant phenotypes, suggesting that AtRFC1 plays an important role in embryo development in Arabidopsis thaliana.
复制因子C（RFC）由一个大亚基和四个小亚基组成，是参与DNA复制、修复机制以及细胞增殖的重要因子。拟南芥RFC的亚基1（AtRFC1）是人类RFC大亚基p140的同源物。已验证了AtRFC1的三个T-DNA插入突变株系，即rfc1-1、rfc1-2和rfc1-3，其插入突变分别位于外显子16、外显子19和启动子区域。这些突变导致胚胎发生缺陷，并导致胚胎和种子败育。将野生型AtRFC1基因转化到rfc1突变等位基因中可恢复突变表型，这表明AtRFC1在拟南芥胚胎发育中起重要作用。
