Daitoku Daisuke, Kurose Tomoyuki, Mori Emiko, Hashimoto Masakazu, Kawamata Seiichi
Department of Anatomy and Histology, Graduate School of Health Sciences, Hiroshima University, Hiroshima, Japan.
Arch Histol Cytol. 2007 Apr;70(1):29-41. doi: 10.1679/aohc.70.29.
An experimental design was developed for morphometric analysis of the subcutaneous connective tissue after the subcutaneous injection of 0.1 ml of saline or a histamine solution (0.01, 0.1 or 1% histamine dihydrochloride in saline). The subcutaneous connective tissue of 4-week-old rats, originally 170.0 +/- 13.6 mum in thickness, swelled 5.2-fold at 15 min, 3.0-fold at 2 h, and 1.2-fold at 6 h after the injection of saline. The total cross sectional area of both blood and lymphatic vessels increased when compared to that at pre-injection (0.0186 +/- 0.0030 mm(2) in 6-mm-long specimen), 1.4-fold at 15 min, 2.2-fold at 2 h, and 1.1-fold at 6 h post-injection, while the total number of these vessels increased 1.1-fold at 2 h. The total cross sectional area of lymphatic vessels (0.0006 +/- 0.0002 mm(2) in 6-mm-long specimen) alone surged 7.7-fold at 15 min, 4.8-fold at 2 h, and 7.3-fold at 6 h. Collagen fibers were respectively highly, moderately, and mildly disorganized in arrangement at 15 min, 2 h, and 6 h after the saline injection. Histamine elicited an earlier, longer, and more pronounced vasodilatation, particularly at high concentrations. The transvascular permeability of Evans blue increased depending on the concentration of histamine. These findings indicate that the subcutaneous connective tissue has the ability to expand and store a considerable amount of fluid and reversibly returns to normal steady-state conditions by increasing fluid excretion into the blood and lymphatic vessels. It was also strongly suggested that the blood vessels are deeply involved in the excretion and volume regulation of the tissue fluid.
为了对皮下注射0.1 ml生理盐水或组胺溶液（盐水中0.01%、0.1%或1%的二盐酸组胺）后的皮下结缔组织进行形态计量分析，设计了一种实验方案。4周龄大鼠的皮下结缔组织，初始厚度为170.0±13.6μm，注射生理盐水后15分钟肿胀5.2倍，2小时肿胀3.0倍，6小时肿胀1.2倍。与注射前相比（6 mm长标本中为0.0186±0.0030 mm²），血液和淋巴管的总横截面积增加，注射后15分钟增加1.4倍，2小时增加2.2倍，6小时增加1.1倍，而这些血管的总数在2小时增加1.1倍。仅淋巴管的总横截面积（6 mm长标本中为0.0006±0.0002 mm²）在15分钟时激增7.7倍，2小时时激增4.8倍，6小时时激增7.3倍。注射生理盐水后15分钟、2小时和6小时，胶原纤维排列分别高度、中度和轻度紊乱。组胺引发更早、更长时间和更明显的血管扩张，尤其是在高浓度时。伊文思蓝的跨血管通透性随组胺浓度增加而增加。这些发现表明，皮下结缔组织有能力扩张并储存大量液体，并通过增加向血液和淋巴管的液体排泄而可逆地恢复到正常稳态。还强烈表明，血管在组织液的排泄和容量调节中起重要作用。
