Mazroa Shireen A, Asker Samar A, Asker Waleed, Abd Ellatif Mohamed
Histology and Cell Biology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
General Surgery Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
Int J Exp Pathol. 2015 Jun;96(3):172-82. doi: 10.1111/iep.12127. Epub 2015 Apr 17.
Polypropylene mesh is commonly used in the treatment of abdominal hernia. Different approaches were addressed to improve their tissue integration and consequently reduce long-term complications. This study aimed to investigate the effect of alpha-lipoic acid (ALA) co-administration on structural and immunohistochemical (IHC) changes in the subcutaneous tissues of the anterior abdominal wall of the adult rat in response to polypropylene mesh implantation. Forty adult male albino rats were divided into: group I (control), group II (receiving ALA), group III (polypropylene mesh implantation) and group IV (mesh implantation + ALA co-administration). After 4 weeks, subcutaneous tissue samples were prepared for light microscopy and IHC study of CD34 as a marker for angiogenesis. In groups I and II rats, positive CD34 expression was demonstrated by IHC reaction, localized to endothelial cells lining small blood vessels. Group III showed an excess inflammatory reaction, deposition of both regular and irregularly arranged collagen fibres around mesh pores and few elastic fibres. CD34-positive was detected not only in cells lining small blood vessels but also in other cells scattered in the connective tissue indicating angiogenesis. In group IV, ALA co-administration resulted in less inflammatory reaction, regular collagen deposition, enhanced elastic fibres synthesis and a significant increase in CD34-positive cells and small blood vessels reflecting improved angiogenesis. ALA co-administration with polypropylene mesh implantation controlled the inflammatory reaction, helped regular collagen deposition, enhanced elastic fibres synthesis and improved angiogenesis in the subcutaneous tissue of anterior abdominal wall of adult albino rats, suggesting a possible role of ALA in optimizing mesh integration in subcutaneous tissue.
聚丙烯网片常用于治疗腹疝。人们采用了不同方法来改善其与组织的整合，从而减少长期并发症。本研究旨在探讨联合给予α-硫辛酸（ALA）对成年大鼠腹壁前皮下组织在植入聚丙烯网片后的结构及免疫组化（IHC）变化的影响。40只成年雄性白化病大鼠被分为：第一组（对照组）、第二组（接受ALA）、第三组（植入聚丙烯网片）和第四组（植入网片+联合给予ALA）。4周后，制备皮下组织样本用于光学显微镜检查以及以CD34作为血管生成标志物的免疫组化研究。在第一组和第二组大鼠中，免疫组化反应显示CD34呈阳性表达，定位于小血管的内皮细胞。第三组显示有过度的炎症反应，在网孔周围有规则和不规则排列的胶原纤维沉积以及少量弹性纤维。不仅在小血管的内皮细胞中检测到CD34阳性，在结缔组织中散在的其他细胞中也检测到，提示有血管生成。在第四组中，联合给予ALA导致炎症反应减轻、胶原沉积规则、弹性纤维合成增强以及CD34阳性细胞和小血管显著增加，反映出血管生成改善。联合给予ALA与聚丙烯网片植入可控制炎症反应，有助于胶原规则沉积，增强弹性纤维合成并改善成年白化病大鼠腹壁前皮下组织的血管生成，提示ALA在优化皮下组织中网片整合方面可能发挥作用。
