Koistinen Kaisa M, Plumed-Ferrer Carme, Lehesranta Satu J, Kärenlampi Sirpa O, von Wright Atte
Institute of Applied Biotechnology, University of Kuopio, Kuopio, Finland.
FEMS Microbiol Lett. 2007 Aug;273(1):12-21. doi: 10.1111/j.1574-6968.2007.00775.x. Epub 2007 Jun 7.
Lactobacillus plantarum is a facultative heterofermentative lactic acid bacterium highly adapted to a wide variety of environments and widely used in food and feed fermentations. Proteomes of two strains of L. plantarum, one isolated from spontaneously fermented cereal-based feed (strain REB1), and the other from white cabbage (strain MLBPL1), were studied to elucidate the strain-specific variation and the physiological changes occurring between the growth (lag, early-exponential, late-exponential and early-stationary) phases of this bacterium when cultivated in a standard rich medium. A total of 231 protein spots were identified by LC-MS/MS. These proteins showed that strain MLBPL1 had more proteins with growth phase-dependent expression than REB1, which possesses a more constant expression profile. The proteins with growth phase-dependent expression in REB1 and MLBPL1 were mainly associated with energy metabolism (glycolysis, phosphoketolase pathway and ribose metabolism), all having preferential expression in the early-exponential phase, confirming the use of different carbohydrates simultaneously. Indication of energy production was also seen in lag and early-stationary phases.
植物乳杆菌是一种兼性异源发酵乳酸菌，高度适应多种环境，广泛应用于食品和饲料发酵。对两株植物乳杆菌的蛋白质组进行了研究，一株从自发发酵的谷物饲料中分离得到（菌株REB1），另一株从白菜中分离得到（菌株MLBPL1），以阐明该细菌在标准丰富培养基中培养时，不同菌株之间的差异以及在生长阶段（延迟期、指数前期、指数后期和稳定前期）发生的生理变化。通过液相色谱-串联质谱法（LC-MS/MS）共鉴定出231个蛋白质斑点。这些蛋白质表明，与具有更恒定表达谱的REB1相比，MLBPL1菌株具有更多与生长阶段相关表达的蛋白质。REB1和MLBPL1中与生长阶段相关表达的蛋白质主要与能量代谢（糖酵解、磷酸酮醇酶途径和核糖代谢）有关，所有这些蛋白质在指数前期均优先表达，这证实了同时使用不同碳水化合物的情况。在延迟期和稳定前期也观察到了能量产生的迹象。
