Laakso Kati, Koskenniemi Kerttu, Koponen Johanna, Kankainen Matti, Surakka Anu, Salusjärvi Tuomas, Auvinen Petri, Savijoki Kirsi, Nyman Tuula A, Kalkkinen Nisse, Tynkkynen Soile, Varmanen Pekka
Research and Development, Valio Ltd, Helsinki, Finland.
Microb Biotechnol. 2011 Nov;4(6):746-66. doi: 10.1111/j.1751-7915.2011.00275.x. Epub 2011 Sep 1.
The growth phase during which probiotic bacteria are harvested and consumed can strongly influence their performance as health-promoting agents. In this study, global transcriptomic and proteomic changes were studied in the widely used probiotic Lactobacillus rhamnosus GG during growth in industrial-type whey medium under strictly defined bioreactor conditions. The expression of 636 genes (P ≤ 0.01) and 116 proteins (P < 0.05) changed significantly over time. Of the significantly differentially produced proteins, 61 were associated with alterations at the transcript level. The most remarkable growth phase-dependent changes occurred during the transition from the exponential to the stationary growth phase and were associated with the shift from glucose fermentation to galactose utilization and the transition from homolactic to mixed acid fermentation. Furthermore, several genes encoding proteins proposed to promote the survival and persistence of L. rhamnosus GG in the host and proteins that directly contribute to human health showed temporal changes in expression. Our results suggest that L. rhamnosus GG has a highly flexible and adaptable metabolism and that the growth stage during which bacterial cells are harvested and consumed should be taken into consideration to gain the maximal benefit from probiotic bacteria.
收获和食用益生菌的生长阶段会强烈影响其作为健康促进剂的性能。在本研究中，我们在严格定义的生物反应器条件下，对工业型乳清培养基中广泛使用的益生菌鼠李糖乳杆菌GG生长过程中的全局转录组和蛋白质组变化进行了研究。636个基因（P≤0.01）和116种蛋白质（P<0.05）的表达随时间发生了显著变化。在显著差异产生的蛋白质中，61种与转录水平的变化相关。最显著的生长阶段依赖性变化发生在从指数生长期向稳定生长期的转变过程中，与从葡萄糖发酵向半乳糖利用的转变以及从同型乳酸发酵向混合酸发酵的转变有关。此外，几个编码被认为可促进鼠李糖乳杆菌GG在宿主中存活和定殖的蛋白质以及直接对人类健康有贡献的蛋白质的基因，其表达也呈现出时间变化。我们的结果表明，鼠李糖乳杆菌GG具有高度灵活且适应性强的代谢，并且在收获和食用细菌细胞时应考虑生长阶段，以从益生菌中获得最大益处。
