Habeeb Fatema, Shakir Eisin, Bradbury Fiona, Cameron Pamela, Taravati Mohamad R, Drummond Allan J, Gray Alexander I, Ferro Valerie A
Faculty of Allied Health, Medical Laboratory Sciences, Kuwait University, P.O. Box 31470, Sulaibikhat-90805, Kuwait.
Methods. 2007 Aug;42(4):315-20. doi: 10.1016/j.ymeth.2007.03.004.
The emergence of antibiotic resistant bacterial strains is a growing problem and is an important concern for patients, physicians, healthcare managers, and policymakers as it results in poorer health and economic outcomes. This has led to an urgent global call for new antimicrobial drugs, particularly from natural resources. We have been studying the antimicrobial properties of the inner leaf gel component of Aloe barbadensis Miller and have used a number of different, simple in vitro assays to establish a scientific basis for the potential use of Aloe vera on a range of clinically relevant bacteria. The bacteria used include Shigella flexneri, Methicillin-Resistant Staphylococcus aureus (MRSA), Enterobacter cloacae and Enterococcus bovis. In this paper, we compare standard methods recommended by the Clinical and Laboratory Standards Institute (CLSI) with a microtitre assay using a metabolic colour indicator Alamar blue. All the techniques described have shown that Aloe vera has an antimicrobial effect, however, the microtitre assay enables high throughput screening, under similar conditions and is less wasteful of plant material.
抗生素耐药细菌菌株的出现是一个日益严重的问题，也是患者、医生、医疗保健管理人员和政策制定者关注的重要问题，因为它会导致较差的健康和经济结果。这引发了全球对新型抗菌药物的迫切需求，尤其是来自自然资源的药物。我们一直在研究库拉索芦荟内叶凝胶成分的抗菌特性，并使用了多种不同的简单体外试验，为芦荟在一系列临床相关细菌上的潜在应用建立科学依据。所使用的细菌包括弗氏志贺菌、耐甲氧西林金黄色葡萄球菌（MRSA）、阴沟肠杆菌和牛肠球菌。在本文中，我们将临床和实验室标准协会（CLSI）推荐的标准方法与使用代谢颜色指示剂阿拉玛蓝的微量滴定法进行了比较。所描述的所有技术都表明芦荟具有抗菌作用，然而，微量滴定法能够在类似条件下进行高通量筛选，并且对植物材料的浪费较少。
