Yosboonruang Atchariya, Phimnuan Preeyawass, Yakaew Swanya, Oonkhanond Bovornlak, Rawangkan Anchalee, Ross Sukunya, Ross Gareth, Viyoch Jarupa
Division of Microbiology and Parasitology, School of Medical Sciences, University of Phayao, Phayao 56000, Thailand.
Department of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Center of Excellence for Innovation in Chemistry, Naresuan University, Phitsanulok 65000, Thailand.
ACS Omega. 2023 May 3;8(19):16824-16832. doi: 10.1021/acsomega.3c00372. eCollection 2023 May 16.
In this study, a biocellulose (BC) sheet containing gel extract (AE) was developed for application in healing chronic wounds, such as diabetic wounds. The BC sheet was produced by and then lyophilized to obtain dried sheets. gel was extracted by precipitation in 35% ammonium sulfate, lyophilized, dried, and incorporated into the BC sheet. The protein content of the AE was 12.32 ± 3.4% w/w, with a molecular weight of ∼20 kDa. The release of TNF-α from lipopolysaccharide-induced RAW264.7 cells was reduced by treatment with AE in a dose-dependent manner. The physicochemical and biological properties of the developed sheet were investigated. Morphological examination of the BC/AE sheet using scanning electron microscopy revealed the 3D construction of nanofibrils, which showed high porosity. The BC/AE sheet exhibited water absorption at 74%, and the release of proteins in the AE reached 97.23% at 4 h. The BC sheet incorporated with proteins in the AE at 283.78 ± 7.7 μg/cm can promote the wound healing in streptozotocin-induced diabetic rats. The recovering skin in diabetic wounds treated with the BC/AE sheet exhibited a normal cell arrangement without fibrosis, as revealed by histological staining. The research findings indicate that the BC/AE sheet has potential for applications in wound dressings.
在本研究中，开发了一种含有芦荟提取物（AE）的生物纤维素（BC）片材，用于治疗慢性伤口，如糖尿病伤口。BC片材通过[具体方法]制备，然后冻干以获得干燥片材。芦荟凝胶通过在35%硫酸铵中沉淀提取，冻干、干燥后掺入BC片材中。AE的蛋白质含量为12.32±3.4%（w/w），分子量约为20 kDa。用AE处理可使脂多糖诱导的RAW264.7细胞中TNF-α的释放呈剂量依赖性降低。对所开发片材的物理化学和生物学特性进行了研究。使用扫描电子显微镜对BC/AE片材进行形态学检查，发现纳米纤维的三维结构，显示出高孔隙率。BC/AE片材的吸水率为74%，AE中蛋白质在4小时时的释放率达到97.23%。以283.78±7.7μg/cm掺入AE中蛋白质的BC片材可促进链脲佐菌素诱导的糖尿病大鼠伤口愈合。组织学染色显示，用BC/AE片材治疗的糖尿病伤口中恢复的皮肤细胞排列正常，无纤维化。研究结果表明，BC/AE片材在伤口敷料方面具有应用潜力。
