Engler Andrea, Aeschlimann André, Simmen Beat R, Michel Beat A, Gay Renate E, Gay Steffen, Sprott Haiko
Center of Experimental Rheumatology, Department of Rheumatology and Institute of Physical Medicine, University Hospital, Gloriastrasse 25, Zurich, Switzerland.
Biochem Biophys Res Commun. 2007 Aug 10;359(4):884-8. doi: 10.1016/j.bbrc.2007.05.178. Epub 2007 Jun 4.
The transient receptor potential vanilloid 1 (TRPV1) is a nonselective cation channel, which is mainly expressed by nociceptive neurons in dorsal root and trigeminal ganglia. However, there is increasing evidence that TRPV1 expression is not limited to primary afferent neurons but that the receptor is expressed in various cell types throughout the body. Here, we demonstrate the expression of TRPV1 in synovial fibroblasts (SF) from patients with symptomatic osteoarthritis (OA) and rheumatoid arthritis (RA). In addition, the mRNA expression of TRPV1 was shown in PBMCs from healthy controls and from OA patients. The presence of TRPV1 was confirmed at the protein level. Stimulation of cultured OA- and RA-SF with the TRPV1 agonist capsaicin led to increased expression of IL-6 mRNA as well as of IL-6 protein in the cell culture supernatants. IL-6 protein expression could be antagonized with capsazepine. Thus, we hypothesize that TRPV1 may play a role in non-neuronal mechanisms that might modulate nociception in symptomatic OA and RA patients.
瞬时受体电位香草酸亚型1（TRPV1）是一种非选择性阳离子通道，主要由背根神经节和三叉神经节中的伤害性神经元表达。然而，越来越多的证据表明，TRPV1的表达并不局限于初级传入神经元，而是在全身各种细胞类型中都有表达。在此，我们证实了TRPV1在有症状骨关节炎（OA）和类风湿关节炎（RA）患者的滑膜成纤维细胞（SF）中的表达。此外，在健康对照者和OA患者的外周血单核细胞（PBMC）中也检测到TRPV1的mRNA表达。在蛋白质水平也证实了TRPV1的存在。用TRPV1激动剂辣椒素刺激培养的OA和RA滑膜成纤维细胞，导致细胞培养上清液中IL-6 mRNA和IL-6蛋白的表达增加。辣椒平可拮抗IL-6蛋白的表达。因此，我们推测TRPV1可能在有症状OA和RA患者中参与调节伤害感受的非神经元机制中发挥作用。
