Scupham Alexandra J
Pre-Harvest Food Safety and Enteric Diseases Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, USA.
Environ Microbiol. 2007 Jul;9(7):1801-9. doi: 10.1111/j.1462-2920.2007.01300.x.
Bromodeoxyuridine, a thymidine analogue that can be incorporated into the DNA of actively dividing cells, has been used in vivo to identify intestinal bacteria that are metabolically active in 3-week-old turkey poults during an acute period of feed withdrawal. Automated ribosomal intergenic spacer analysis was used to identify amplicons unique to animals subjected to feed withdrawal. One amplicon was unique to fasted birds while two amplicons were present in 60% of fasted birds and absent in all fed birds. Sequence analysis of 16S ribosomal genes indicated the caecal communities of all birds were dominated by Clostridiaceae while also harbouring low levels of metabolically active gamma-proteobacteria and Bacteroides. Twenty per cent of clones from the fasted animals were identified as belonging to the genus Papillibacter, suggesting these microbes may be specifically dividing in response to environmental conditions present in the caeca of fasted birds.
溴脱氧尿苷是一种胸腺嘧啶类似物，可掺入活跃分裂细胞的DNA中，已在体内用于识别3周龄火鸡雏在急性禁食期具有代谢活性的肠道细菌。利用自动核糖体基因间隔区分析来鉴定禁食动物特有的扩增子。一个扩增子是禁食鸟类所特有的，而两个扩增子存在于60%的禁食鸟类中，所有喂食鸟类中均不存在。16S核糖体基因的序列分析表明，所有鸟类的盲肠群落均以梭菌科为主，同时也含有低水平的代谢活跃的γ-变形菌和拟杆菌。来自禁食动物的20%的克隆被鉴定属于乳头杆菌属，这表明这些微生物可能因禁食鸟类盲肠中存在的环境条件而特异性分裂。
