Suchodolski Jan S, Camacho Jennifer, Steiner Jörg M
Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843-4474, USA.
FEMS Microbiol Ecol. 2008 Dec;66(3):567-78. doi: 10.1111/j.1574-6941.2008.00521.x. Epub 2008 Jun 16.
The study aim was to describe the diversity of the intraluminal intestinal microbial community in dogs by direct sequence analysis of the 16S rRNA gene. Intestinal content was collected from the duodenum, jejunum, ileum, and colon from six healthy dogs. Bacterial 16S rRNA gene was amplified with universal bacterial primers. Amplicons were ligated into cloning vectors and near-full-length 16S rRNA gene inserts were analyzed. From a total of 864 clones analyzed, 106 nonredundant 16S rRNA gene sequences were identified. Forty-two (40%) sequences showed<98% sequence similarity to 16S rRNA gene sequences reported previously. Operation taxonomic units were classified into four phyla: Firmicutes, Fusobacteria, Bacteroidetes, and Proteobacteria. Clostridiales predominated in the duodenum (40% of clones) and jejunum (39%), and were highly abundant in the ileum (25%) and colon (26%). Sequences affiliated with Clostridium cluster XI and Clostridium cluster XIVa dominated in the proximal small intestine and colon, respectively. Fusobacteriales and Bacteroidales were the most abundant bacterial order in the ileum (33%) and colon (30%). Enterobacteriales were more commonly observed in the small intestine than in the colon. Lactobacillales occurred commonly in all parts of the intestine.
本研究旨在通过对16S rRNA基因进行直接序列分析，描述犬肠道腔内微生物群落的多样性。从6只健康犬的十二指肠、空肠、回肠和结肠采集肠内容物。用通用细菌引物扩增细菌16S rRNA基因。将扩增子连接到克隆载体中，并分析近全长16S rRNA基因插入片段。在总共分析的864个克隆中，鉴定出106个非冗余的16S rRNA基因序列。42个（40%）序列与先前报道的16S rRNA基因序列的相似性<98%。操作分类单元分为四个门：厚壁菌门、梭杆菌门、拟杆菌门和变形菌门。梭菌目在十二指肠（占克隆的40%）和空肠（39%）中占主导地位，在回肠（25%）和结肠（26%）中含量也很高。分别与XI群梭菌和XIVa群梭菌相关的序列在近端小肠和结肠中占主导地位。梭杆菌目和拟杆菌目是回肠（33%）和结肠（30%）中含量最丰富的细菌目。肠杆菌目在小肠中比在结肠中更常见。乳杆菌目在肠道各部位均普遍存在。
