在骨巨细胞瘤中检测到端粒关联和持续的生长因子过表达。
Telomeric associations and consistent growth factor overexpression detected in giant cell tumor of bone.
作者信息
Schwartz H S, Butler M G, Jenkins R B, Miller D A, Moses H L
机构信息
Department of Orthopedics, Vanderbilt University, Nashville, Tennessee.
出版信息
Cancer Genet Cytogenet. 1991 Oct 15;56(2):263-76. doi: 10.1016/0165-4608(91)90179-x.
Tumor specimens from 15 patients with giant cell tumor (GCT) of bone were cytogenetically analyzed. A subset of five individuals had tumor cells harvested and polyadenylated RNA isolated. Multiple Northern blots were performed utilizing radiolabeled probes for the growth factors TGF beta 1, TGF beta 2, TGF beta 3, and TGF alpha (TGF, transforming growth factor). RNAs from other types of neoplasms and nonneoplastic cells were examined as controls. The most consistent cytogenetic abnormality detected involved multiple telomeric associations (TAs), most frequently involving the terminus of the long arm of chromosome 19 (19q). Northern blot analysis revealed a consistent expression of TGF beta 1 and TGF beta 2 with an inconsistent mRNA expression for the other TGFs. There was a relative overexpression of mRNA for TGF beta 2. The gene location for TGF beta 1 is near the 19q terminus and thus it is speculated that TGF beta may play a role in the neoplastic transformation of GCT.
对15例骨巨细胞瘤（GCT）患者的肿瘤标本进行了细胞遗传学分析。从其中5例患者中采集肿瘤细胞并分离出聚腺苷酸化RNA。使用针对生长因子转化生长因子β1（TGFβ1）、转化生长因子β2（TGFβ2）、转化生长因子β3（TGFβ3）和转化生长因子α（TGFα）的放射性标记探针进行了多次Northern印迹分析。作为对照，检测了来自其他类型肿瘤和非肿瘤细胞的RNA。检测到的最一致的细胞遗传学异常涉及多个端粒联合（TA），最常见的是涉及19号染色体长臂末端（19q）。Northern印迹分析显示TGFβ1和TGFβ2表达一致，而其他TGF的mRNA表达不一致。TGFβ2的mRNA有相对过表达。TGFβ1的基因位置靠近19q末端，因此推测TGFβ可能在GCT的肿瘤转化中起作用。
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