Kodama K, Doi O, Higashiyama M, Mori Y, Horai T, Tateishi R, Aoki Y, Misawa S
Department of Thoracic Surgery, Kyoto Prefectural University of Medicine, Japan.
Cancer Genet Cytogenet. 1991 Nov;57(1):19-30. doi: 10.1016/0165-4608(91)90185-w.
A new human Ewing's sarcoma cell line (CADO-ES1) was established from the malignant pleural effusion of a 19-year-old woman. These cells grew both anchorage dependently and anchorage independently. When cultured in bacteriologic dishes, they grew as tightly packed multicellular tumor spheroids; they were also capable of proliferating in soft agar. Flow cytometric DNA analysis demonstrated a nearly diploid DNA content (DNA index = 0.902). Chromosomal studies of cultured cells showed an isodicentric chromosome 8 in all examined cells, but t(11;22)(q24;q12), a translocation reported previously in Ewing's sarcoma, was not detected. Under normal culture conditions, no morphologic evidence of neural differentiation was detected. In addition, immunocytochemical studies showed that vimentin was intensely positive, whereas neurofilament (NF) and neuron-specific enolase (NSE) were weakly positive. Treatment with cyclic AMP (cAMP) induced pronounced morphologic evidence of neural differentiation and strong expression of NF in cultured cells. S-100 protein, glial fibrillary acidic protein (GFAP), desmin, cytokeratin, and epithelial membrane antigen were not detected immunohistochemically in either untreated or cAMP-treated cells, however. These data suggest that this cell line is derived from a highly undifferentiated neural cell with high chromosomal clonality, differentiating into neural features under certain conditions.
一株新的人尤因肉瘤细胞系（CADO-ES1）从一名19岁女性的恶性胸腔积液中建立。这些细胞既能贴壁生长，也能非贴壁生长。在细菌培养皿中培养时，它们长成紧密堆积的多细胞肿瘤球体；它们也能够在软琼脂中增殖。流式细胞术DNA分析显示DNA含量接近二倍体（DNA指数 = 0.902）。对培养细胞的染色体研究表明，所有检测细胞中均有一条等臂染色体8，但未检测到先前在尤因肉瘤中报道的t(11;22)(q24;q12)易位。在正常培养条件下，未检测到神经分化的形态学证据。此外，免疫细胞化学研究表明波形蛋白呈强阳性，而神经丝（NF）和神经元特异性烯醇化酶（NSE）呈弱阳性。用环磷酸腺苷（cAMP）处理可诱导培养细胞出现明显的神经分化形态学证据，并使NF强烈表达。然而，在未处理或cAMP处理的细胞中，免疫组织化学均未检测到S-100蛋白、胶质纤维酸性蛋白（GFAP）、结蛋白、细胞角蛋白和上皮膜抗原。这些数据表明，该细胞系来源于具有高染色体克隆性的高度未分化神经细胞，在某些条件下可分化为神经特征。
