Gerharz C D, Moll R, Ramp U, Mellin W, Gabbert H E
Department of Pathology, University of Mainz, FRG.
Int J Cancer. 1990 Jan 15;45(1):143-52. doi: 10.1002/ijc.2910450126.
A new permanent cell line (GRU-1) derived from the lymph-node metastasis of a human epithelioid sarcoma was established in tissue culture. Immunohistochemically, the original tumor had exhibited an intriguing potential for multidirectional differentiation with features of mesenchymal, epithelial and neural differentiation, evidenced by the co-expression of vimentin, cytokeratins and neurofilament proteins, respectively. This capability for multidirectional differentiation was fully preserved in the cultured cells. GRU-1 tumor cells proved to be uniformly positive for vimentin and a considerable proportion of the tumor cells exhibited a positive reaction for cytokeratins and neurofilament proteins. The neural markers neuron-specific enolase (NSE) and synaptophysin were observed in a small proportion of GRU-1 cells. Ultrastructurally, GRU-1 cells showed desmoplastic activity in vitro, being enmeshed by collagen fibrils. DNA distribution, as studied by flow cytophotometry, revealed DNA-diploidy (DNA index = 1) and a G0/G1-proportion of 70.5%. After heterotransplantation in nude mice, GRU-1 tumor cells expressed vimentin and cytokeratin only, whereas the neural markers could not be further demonstrated.
通过组织培养建立了一种源自人上皮样肉瘤淋巴结转移灶的新的永久细胞系（GRU-1）。免疫组织化学分析显示，原发肿瘤具有多向分化潜能，表现为间充质、上皮和神经分化特征，分别通过波形蛋白、细胞角蛋白和神经丝蛋白的共表达得以证实。这种多向分化能力在培养细胞中得到了充分保留。GRU-1肿瘤细胞被证实波形蛋白均呈阳性，相当比例的肿瘤细胞对细胞角蛋白和神经丝蛋白呈阳性反应。在一小部分GRU-1细胞中观察到神经标志物神经元特异性烯醇化酶（NSE）和突触素。超微结构观察显示，GRU-1细胞在体外表现出促纤维增生活性，被胶原纤维包裹。通过流式细胞光度术研究DNA分布，结果显示为DNA二倍体（DNA指数=1），G0/G1期比例为70.5%。将GRU-1肿瘤细胞异种移植到裸鼠体内后，仅表达波形蛋白和细胞角蛋白，而神经标志物则无法进一步检测到。
