Mose Tina, Knudsen Lisbeth E, Hedegaard Morten, Mortensen Gerda K
Department of Environmental and Occupational Health, Institute of Public Health, University of Copenhagen, Denmark.
Int J Toxicol. 2007 May-Jun;26(3):221-9. doi: 10.1080/10915810701352721.
The transplacental passage of monomethylphtalate (mMP) and mono (2-ethylhexyl) phthalate (mEHP) was studied using an ex vivo placental perfusion model with simultaneous perfusion of fetal and maternal circulation in a single cotyledon. Umbilical cord blood and placental tissue collected both before and after perfusion were also analyzed. Placentas were obtained immediately after elective cesarean section and dually perfused in a recirculation system. mMP or mEHP was added to maternal perfusion medium to obtain concentrations at 10 and 25 microg/L, respectively. The placental transfer was followed analyzing samples from fetal and maternal perfusion media by liquid chromatography-mass spectrometry-mass spectrometry (LC-MS-MS). Four perfusions with mMP indicated a slow transplacental transfer, with a feto-maternal ratio (FM ratio) of 0.30 +/- 0.03 after 150 min of perfusion. Four perfusions with mEHP indicated a very slow or nonexisting placental transfer. mEHP was only detected in fetal perfusion media from two perfusions, giving rise to FM ratios of 0.088 and 0.20 after 150 min of perfusion. Detectable levels of mMP, mEHP, monoethylphthalate (mEP), and monobutylphthalate were found in tissue. Higher tissue levels of mMP after perfusions with mMP compared to perfusions with mEHP suggest an accumulation of mMP during perfusion. No tendency for accumulation of mEHP was observed during perfusions with mEHP compared to perfusions with mMP. Detectable levels of mEHP and mEP were found in umbilical cord plasma samples. mMP and possibly other short-chained phthalate monoesters in maternal blood can cross the placenta by slow transfer, whereas the results indicate no placental transfer of mEHP. Further studies are recommended.
使用离体胎盘灌注模型，在单个子叶中同时灌注胎儿和母体循环，研究了单甲基邻苯二甲酸酯（mMP）和单（2-乙基己基）邻苯二甲酸酯（mEHP）的胎盘转运情况。还分析了灌注前后收集的脐带血和胎盘组织。在择期剖宫产术后立即获取胎盘，并在再循环系统中进行双重灌注。将mMP或mEHP添加到母体灌注培养基中，使其浓度分别达到10和25微克/升。通过液相色谱-质谱-质谱联用仪（LC-MS-MS）分析胎儿和母体灌注培养基中的样品，追踪胎盘转运情况。四次mMP灌注显示胎盘转运缓慢，灌注150分钟后胎儿与母体的比例（FM比例）为0.30±0.03。四次mEHP灌注显示胎盘转运非常缓慢或不存在。仅在两次灌注的胎儿灌注培养基中检测到mEHP，灌注150分钟后FM比例分别为0.088和0.20。在组织中发现了可检测水平的mMP、mEHP、单乙基邻苯二甲酸酯（mEP）和单丁基邻苯二甲酸酯。与mEHP灌注相比，mMP灌注后组织中mMP水平更高，表明灌注过程中mMP会积累。与mMP灌注相比，mEHP灌注过程中未观察到mEHP积累的趋势。在脐带血浆样本中发现了可检测水平的mEHP和mEP。母体血液中的mMP以及可能其他短链邻苯二甲酸单酯可通过缓慢转运穿过胎盘，而结果表明mEHP无胎盘转运。建议进一步研究。
