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Structural basis for cofactor-independent dioxygenation of N-heteroaromatic compounds at the alpha/beta-hydrolase fold.α/β-水解酶折叠中非依赖辅因子的杂芳族 N-化合物双氧代的结构基础。
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Pseudomonas 2007.假单胞菌2007年
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本文引用的文献

1
Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
Crystallization and diffraction data of 1H-3-hydroxy-4-oxoquinoline 2,4-dioxygenase: a cofactor-free oxygenase of the alpha/beta-hydrolase family.1H - 3 - 羟基 - 4 - 氧代喹啉2,4 - 双加氧酶的结晶与衍射数据：α/β - 水解酶家族的一种无辅因子加氧酶
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1;63(Pt 5):378-81. doi: 10.1107/S1744309107013760. Epub 2007 Apr 6.
3
Dioxygenases without requirement for cofactors and their chemical model reaction: compulsory order ternary complex mechanism of 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase involving general base catalysis by histidine 251 and single-electron oxidation of the substrate dianion.无需辅因子的双加氧酶及其化学模型反应：1H-3-羟基-4-氧代喹哪啶2,4-双加氧酶的强制顺序三元复合物机制，涉及组氨酸251的一般碱催化和底物二价阴离子的单电子氧化。
Biochemistry. 2004 Nov 16;43(45):14485-99. doi: 10.1021/bi048735u.
4
Breaking good resolutions with ARP/wARP.用ARP/wARP打破良好的决心。
J Synchrotron Radiat. 2004 Jan 1;11(Pt 1):56-9. doi: 10.1107/s090904950302394x. Epub 2003 Nov 28.
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Generation, representation and flow of phase information in structure determination: recent developments in and around SHARP 2.0.结构测定中相位信息的生成、表示及流动：SHARP 2.0及其周边的最新进展
Acta Crystallogr D Biol Crystallogr. 2003 Nov;59(Pt 11):2023-30. doi: 10.1107/s0907444903017694. Epub 2003 Oct 23.
6
Anaerobic enzyme.substrate structures provide insight into the reaction mechanism of the copper-dependent quercetin 2,3-dioxygenase.厌氧酶-底物结构有助于深入了解铜依赖性槲皮素2,3-双加氧酶的反应机制。
Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16625-30. doi: 10.1073/pnas.262506299. Epub 2002 Dec 16.
7
Oxygenases without requirement for cofactors or metal ions.无需辅因子或金属离子的加氧酶。
Appl Microbiol Biotechnol. 2002 Nov;60(3):243-57. doi: 10.1007/s00253-002-1123-4. Epub 2002 Oct 12.
8
Substructure solution with SHELXD.使用SHELXD进行亚结构解析。
Acta Crystallogr D Biol Crystallogr. 2002 Oct;58(Pt 10 Pt 2):1772-9. doi: 10.1107/s0907444902011678. Epub 2002 Sep 28.
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Alpha/beta hydrolase fold enzymes: the family keeps growing.α/β水解酶折叠酶家族：不断发展壮大。
Curr Opin Struct Biol. 1999 Dec;9(6):732-7. doi: 10.1016/s0959-440x(99)00037-8.
10
Bacterial 2,4-dioxygenases: new members of the alpha/beta hydrolase-fold superfamily of enzymes functionally related to serine hydrolases.细菌2,4-双加氧酶：与丝氨酸水解酶功能相关的α/β水解酶折叠超家族的新成员。
J Bacteriol. 1999 Sep;181(18):5725-33. doi: 10.1128/JB.181.18.5725-5733.1999.

嗜硝化节杆菌Rü61a来源的1H-3-羟基-4-氧代喹哪啶2,4-双加氧酶的结晶及初步X射线分析：α/β-水解酶折叠超家族中一种不含辅因子的双加氧酶

Crystallization and preliminary X-ray analysis of 1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase from Arthrobacter nitroguajacolicus Rü61a: a cofactor-devoid dioxygenase of the alpha/beta-hydrolase-fold superfamily.

作者信息

Steiner Roberto A, Frerichs-Deeken Ursula, Fetzner Susanne

机构信息

Randall Division of Cell and Molecular Biophysics, King's College London, New Hunt's House, Guy's Campus, London SE1 1UL, England.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1;63(Pt 5):382-5. doi: 10.1107/S174430910701353X. Epub 2007 Apr 6.

DOI:10.1107/S174430910701353X
PMID:17565176
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2335005/
Abstract

1H-3-Hydroxy-4-oxoquinaldine 2,4-dioxygenase (HOD) is a cofactor-devoid dioxygenase that is involved in the anthranilate pathway of quinaldine degradation. HOD has been proposed to belong to the alpha/beta-hydrolase-fold superfamily of enzymes. N-terminally His6-tagged HOD has been crystallized by the hanging-drop vapour-diffusion method using sodium/potassium tartrate as a precipitant and CuCl2 as an additive. The structure was solved by the single anomalous dispersion (SAD) technique using data collected to 3.5 A resolution at the Cu absorption peak wavelength. The crystals belong to the primitive tetragonal space group P4(3)2(1)2, with unit-cell parameters a = b = 153.788, c = 120.872 A.

摘要

1H-3-羟基-4-氧代喹哪啶2,4-双加氧酶（HOD）是一种不含辅因子的双加氧酶，参与喹哪啶降解的邻氨基苯甲酸途径。有人提出HOD属于α/β-水解酶折叠超家族的酶。N端带有His6标签的HOD已通过悬滴气相扩散法结晶，使用酒石酸钠/钾作为沉淀剂，CuCl2作为添加剂。该结构通过在Cu吸收峰波长下收集到3.5埃分辨率的数据，采用单波长反常散射（SAD）技术解析。晶体属于原始四方空间群P4(3)2(1)2，晶胞参数a = b = 153.788，c = 120.872埃。