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Novel versatile cryoprotectants for heavy-atom derivatization of protein crystals.用于蛋白质晶体重原子衍生化的新型多功能冷冻保护剂。
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In vitro alginate polymerization and the functional role of Alg8 in alginate production by Pseudomonas aeruginosa.体外藻酸盐聚合以及Alg8在铜绿假单胞菌藻酸盐产生中的功能作用。
Appl Environ Microbiol. 2006 Jan;72(1):298-305. doi: 10.1128/AEM.72.1.298-305.2006.
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J Bacteriol. 2005 Dec;187(24):8375-84. doi: 10.1128/JB.187.24.8375-8384.2005.
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Alginate lyase (AlgL) activity is required for alginate biosynthesis in Pseudomonas aeruginosa.铜绿假单胞菌中藻酸盐生物合成需要藻酸盐裂解酶（AlgL）的活性。
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荧光假单胞菌AlgK的表达、纯化、结晶及初步X射线分析

Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas fluorescens AlgK.

作者信息

Keiski Carrie-Lynn, Yip Patrick, Robinson Howard, Burrows Lori L, Howell P Lynne

机构信息

Program in Molecular Structure and Function, Research Institute, Hospital for Sick Children, 555 University Avenue, Toronto, Ontario M5G 1X8, Canada.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 May 1;63(Pt 5):415-8. doi: 10.1107/S1744309107016880. Epub 2007 Apr 14.

DOI:10.1107/S1744309107016880
PMID:17565185
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2335008/
Abstract

AlgK is an outer-membrane lipoprotein involved in the biosynthesis of alginate in Pseudomonads and Azotobacter vinelandii. A recombinant form of Pseudomonas fluorescens AlgK with a C-terminal polyhistidine affinity tag has been expressed and purified from the periplasm of Escherichia coli cells and diffraction-quality crystals of AlgK have been grown using the hanging-drop vapour-diffusion method. The crystals grow as flat plates with unit-cell parameters a = 79.09, b = 107.85, c = 119.15 A, beta = 96.97 degrees. The crystals exhibit the symmetry of space group P2(1) and diffract to a minimum d-spacing of 2.5 A at Station X29 of the National Synchrotron Light Source, Brookhaven National Laboratory. On the basis of the Matthews coefficient (V(M) = 2.53 A3 Da(-1)), four protein molecules are estimated to be present in the asymmetric unit.

摘要

AlgK是一种外膜脂蛋白，参与假单胞菌和棕色固氮菌中藻酸盐的生物合成。带有C端多组氨酸亲和标签的重组荧光假单胞菌AlgK已在大肠杆菌细胞周质中表达并纯化，且已使用悬滴气相扩散法培养出了AlgK的衍射质量晶体。晶体生长为平板状，晶胞参数为a = 79.09、b = 107.85、c = 119.15 Å，β = 96.97°。这些晶体具有空间群P2(1)的对称性，在布鲁克海文国家实验室的国家同步辐射光源X29站上衍射到最小d间距为2.5 Å。根据马修斯系数（V(M) = 2.53 Å3 Da(-1)），估计不对称单位中存在四个蛋白质分子。