Girard B, Cloutier M, Wilson D J, Clokie C M L, Miller R J D, Wilson B C
Department of Medical Biophysics, Ontario Cancer Institute and University of Toronto, Ontario, Canada.
Lasers Surg Med. 2007 Jun;39(5):458-67. doi: 10.1002/lsm.20493.
This study investigated the healing of femtosecond laser created wounds in an animal model.
We have assessed the healing of critical size wounds in mice calvaria using three different wounding techniques: carbide bur, diamond end-cutting bur, and ultrafast femtosecond laser, and in the presence or absence of bone morphogenetic protein-7 (BMP). Wound closure was examined using microcomputerized tomography at 3, 6, 9, and 12 weeks.
Results have shown partial closure at up to 12 weeks with all techniques that did not involve the use of BMP, with the least closure noted in the laser groups as suggested by two-dimensional radiographic analysis. Bone volume measurements appeared slightly lower for the laser than for the mechanical groups, however statistically significant differences were seen only at week 6. No significant differences in closure were noted for the different methods in the BMP treated groups.
Femtosecond laser cutting demonstrated an unsurpassed precision when compared to mechanical instruments. The addition of BMP led to very rapid healing with complete closure seen as early as 3 weeks and overcomes any potential healing delays that may arise from laser tissue cutting.
本研究在动物模型中探究了飞秒激光造成的伤口愈合情况。
我们使用三种不同的造伤技术评估了小鼠颅骨临界尺寸伤口的愈合情况：硬质合金牙钻、金刚石端切牙钻和超快飞秒激光，并在有或无骨形态发生蛋白-7（BMP）的情况下进行。在3、6、9和12周时使用微型计算机断层扫描检查伤口闭合情况。
结果显示，所有未使用BMP的技术在12周时均有部分闭合，二维放射学分析表明激光组的闭合程度最低。激光组的骨体积测量值似乎略低于机械组，但仅在第6周时观察到统计学上的显著差异。在BMP治疗组中，不同方法的闭合情况没有显著差异。
与机械器械相比，飞秒激光切割显示出无与伦比的精度。添加BMP可导致非常快速的愈合，早在3周时就可见完全闭合，并克服了激光组织切割可能引起的任何潜在愈合延迟。
