飞秒激光骨消融诱导骨髓基质细胞向成骨细胞分化
Osteoblast differentiation of bone marrow stromal cells by femtosecond laser bone ablation.
作者信息
Zheng Jianqiao, Zhang Xinyue, Zhang Yaopeng, Yuan Fusong
机构信息
Center of Digital Dentistry, Peking University School and Hospital of Stomatology, Beijing, China.
Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, China.
出版信息
Biomed Opt Express. 2020 Jan 14;11(2):885-894. doi: 10.1364/BOE.383721. eCollection 2020 Feb 1.
Abstract
This study examines the osteogenic effect of femtosecond laser bone ablation on bone mesenchymal stromal cells (BMSCs). Three-week old Sprague-Dawley (SD) rats were selected for experiments. Right tibias were ablated by a 10-W femtosecond laser (treated group), whereas left tibias were not subjected to laser ablation (control group). After ablation, BMSCs of both tibias were cultured and purified separately. Cell proliferation was then analyzed, as well as the expressions of RNA and several proteins (alkaline phosphatase (ALP), runt-related transcription factor 2 (RUNX2) and osteocalcin (OCN)). The results suggest that femtosecond laser ablation promotes the differentiation of BMSCs and up-regulates the expression of ALP, RUNX2, and OCN, without affecting BMSC proliferation.
摘要
本研究考察飞秒激光骨消融对骨髓间充质干细胞（BMSCs）的成骨作用。选用3周龄的Sprague-Dawley（SD）大鼠进行实验。用10瓦飞秒激光对右侧胫骨进行消融（治疗组），而左侧胫骨不进行激光消融（对照组）。消融后，分别培养和纯化两侧胫骨的BMSCs。然后分析细胞增殖情况，以及RNA和几种蛋白质（碱性磷酸酶（ALP）、 runt相关转录因子2（RUNX2）和骨钙素（OCN））的表达。结果表明，飞秒激光消融可促进BMSCs的分化，并上调ALP、RUNX2和OCN的表达，而不影响BMSCs的增殖。
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