使用高重复率光纤激光源的飞秒激光骨消融
Femtosecond laser bone ablation with a high repetition rate fiber laser source.
作者信息
Mortensen Luke J, Alt Clemens, Turcotte Raphaël, Masek Marissa, Liu Tzu-Ming, Côté Daniel C, Xu Chris, Intini Giuseppe, Lin Charles P
机构信息
Department of Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, Massachusetts, USA ; Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Advanced Microscopy Program, Center for Systems Biology and Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
出版信息
Biomed Opt Express. 2014 Dec 5;6(1):32-42. doi: 10.1364/BOE.6.000032. eCollection 2015 Jan 1.
Femtosecond laser pulses can be used to perform very precise cutting of material, including biological samples from subcellular organelles to large areas of bone, through plasma-mediated ablation. The use of a kilohertz regenerative amplifier is usually needed to obtain the pulse energy required for ablation. This work investigates a 5 megahertz compact fiber laser for near-video rate imaging and ablation in bone. After optimization of ablation efficiency and reduction in autofluorescence, the system is demonstrated for the in vivo study of bone regeneration. Image-guided creation of a bone defect and longitudinal evaluation of cellular injury response in the defect provides insight into the bone regeneration process.
飞秒激光脉冲可用于通过等离子体介导的烧蚀对材料进行非常精确的切割，包括从亚细胞器到大面积骨骼的生物样本。通常需要使用千赫兹再生放大器来获得烧蚀所需的脉冲能量。这项工作研究了一种用于骨组织近视频速率成像和烧蚀的5兆赫兹紧凑型光纤激光器。在优化烧蚀效率并降低自发荧光后，该系统被用于骨再生的体内研究。图像引导下骨缺损的创建以及对缺损处细胞损伤反应的纵向评估为骨再生过程提供了深入了解。
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