Ji Yu-xin, Zhang Ping, Chen Wei-min, Zhu Sheng-rong, Tao Xue-jin
Center of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2007 Mar;42(3):180-3.
To investigate the inhibition of telomerase activity and cellular proliferation in tongue cancer TCCA-8113 cell lines by antisense human tankyrase-1 RNA treatment, and explore the possibility of the tankyrase-1 as a target of gene therapy for tongue cancer.
The replication deficient retrovirus expressing tankyrase-1 antisense RNA was constructed to infect the TCCA-8113 cells. Tankyrase-1 expression was examined by RT-PCR. Telomerase activity was assayed by telomerase repeat amplification protocol (TRAP). Cell proliferation was investigated by cellular growth curve. Cellular apoptosis was detected by flow cytometry method and invert microscope.
Tankyrase-1 expression and telomerase activity of tongue cancer TCCA-8113 cells were significantly inhibited. There was G(1)-S phase arrest when TCCA-8113 cells were treated with antisense tankyrase-1 transduction. Cellular proliferation was arrested, and cellular apoptosis occurred after antisense tankyrase transduction.
The transduction of antisense tankyrase-1 by retroviral vector can significantly inhibit the tankyrase-1 expression and telomerase activity of tongue cancer TCCA-8113 cell lines, and arrest the cellular proliferation and promote cellular apoptosis. The tankyrase may be a potential target of gene therapy for tongue cancer.
通过反义人端粒酶相关蛋白-1 RNA处理，研究其对舌癌TCCA - 8113细胞系端粒酶活性及细胞增殖的抑制作用，并探讨端粒酶相关蛋白-1作为舌癌基因治疗靶点的可能性。
构建表达端粒酶相关蛋白-1反义RNA的复制缺陷型逆转录病毒，感染TCCA - 8113细胞。采用逆转录聚合酶链反应（RT-PCR）检测端粒酶相关蛋白-1的表达。通过端粒酶重复序列扩增法（TRAP）检测端粒酶活性。采用细胞生长曲线研究细胞增殖情况。通过流式细胞术和倒置显微镜检测细胞凋亡。
舌癌TCCA - 8113细胞的端粒酶相关蛋白-1表达及端粒酶活性均受到显著抑制。用反义端粒酶相关蛋白-1转导处理TCCA - 8113细胞后出现G(1)-S期阻滞。反义端粒酶相关蛋白转导后细胞增殖受到抑制，并发生细胞凋亡。
逆转录病毒载体介导的反义端粒酶相关蛋白-1转导可显著抑制舌癌TCCA - 8113细胞系的端粒酶相关蛋白-1表达及端粒酶活性，抑制细胞增殖并促进细胞凋亡。端粒酶相关蛋白可能是舌癌基因治疗的一个潜在靶点。
