Connor Kathleen M, Fontaine Michael C, Rudge Karen, Baird Graham J, Donachie William
Moredun Research Institute, International Research Centre, Pentlands Science Park, Bush Loan, Penicuik, Midlothian EH26 0PZ, Scotland, United Kingdom.
Vet Res. 2007 Jul-Aug;38(4):613-23. doi: 10.1051/vetres:2007013. Epub 2007 Jun 13.
Caseous lymphadenitis (CLA) is a chronic, suppurative disease, with a worldwide distribution, caused by Corynebacterium pseudotuberculosis. The clinical manifestation of CLA is known to vary between different countries, and has been postulated to be due to differences in the strains present in these countries. Forty-two sheep and goat isolates of C. pseudotuberculosis from Australia, Canada, Eire, The Netherlands and Northern Ireland were characterized by pulsed-field gel electrophoresis (PFGE), biotyping, antimicrobial susceptibility, and production of phospholipase D. The PFGE-determined genotypes of this multicentric collection were then compared with representative ovine and caprine isolates from a previously published panel of PFGE profiles of United Kingdom isolates. Digestion with SfiI generated 16-18 bands in the 48.5 and 290 kb range, and differentiated four distinct pulsotypes amongst the 36 ovine and 6 caprine strains which displayed remarkable homogeneity. Based on these results, it would appear that the genome of C. pseudotuberculosis is highly conserved, irrespective of the country of strain origin.
干酪性淋巴结炎（CLA）是一种由伪结核棒状杆菌引起的慢性化脓性疾病，在全球范围内均有分布。已知CLA的临床表现因不同国家而异，据推测这是由于这些国家存在的菌株差异所致。采用脉冲场凝胶电泳（PFGE）、生物分型、抗菌药敏试验以及磷脂酶D的产生情况，对来自澳大利亚、加拿大、爱尔兰、荷兰和北爱尔兰的42株伪结核棒状杆菌绵羊和山羊分离株进行了特征分析。然后，将该多中心收集菌株经PFGE确定的基因型与先前发表的一组英国分离株PFGE图谱中的代表性绵羊和山羊分离株进行了比较。用SfiI酶切在48.5至290 kb范围内产生了16 - 18条带，并在36株绵羊菌株和6株山羊菌株中区分出四种不同的脉冲型，这些菌株表现出显著的同质性。基于这些结果，似乎伪结核棒状杆菌的基因组高度保守，无论菌株的来源国是哪里。
