CAR, HAS, Institute for Veterinary Medical Research, P.O. Box 18, Budapest 1581, Hungary.
Government Office for Borsod-Abaúj-Zemplén County, Vologda U. 1, Miskolc 3525, Hungary.
Biomed Res Int. 2017;2017:1762162. doi: 10.1155/2017/1762162. Epub 2017 Oct 11.
Thirteen biotype ovis strains isolated from clinical cases of caseous lymphadenitis in Hungary were characterised using multilocus sequencing and their phylogenetic comparison was carried out on the basis of four housekeeping genes (EL1, B, K, and A). The in silico analysis of the 16 frequently studied housekeeping genes showed that strains could be readily distinguished from and strains; however, sequences of the same genes in the two biotypes of the were highly similar; the heterogeneity values were low. Genes K, B, EL1, and A showed marked genetic variation within , and strains of the two biotypes of C could be differentiated. Analysis of the individual genes showed a fairly conservative nature of biotype ovis strains. The greatest genetic differentiation was seen in the and genes and concatenations of these two genes were very useful in the genetic separation of the studied strains.
从匈牙利干酪样淋巴结炎临床病例中分离出的 13 株绵羊生物型菌株，采用多位点测序技术进行鉴定，并基于 4 个管家基因（EL1、B、K 和 A）进行系统发育比较。对 16 个经常研究的管家基因的计算机分析表明，这些菌株很容易与 和 菌株区分开来；然而，这两种生物型的 菌株的相同基因序列非常相似；异质性值较低。K、B、EL1 和 A 基因在 内表现出明显的遗传变异，并且可以区分两种生物型的 C 菌株。对单个基因的分析表明，绵羊生物型菌株具有相当保守的性质。在 和 基因中观察到最大的遗传分化，并且这两个基因的拼接在研究菌株的遗传分离中非常有用。
