巴西珊瑚蛇（小盾鼻蛇属）毒液的比较色谱分析。

Comparative chromatography of Brazilian coral snake (Micrurus) venoms.

da Silva Júnior N J, Griffin P R, Aird S D

Centro de Estudos e Pesquisas Biológicas, Universidade Católica de Goiás, Brazil.

Comp Biochem Physiol B. 1991;100(1):117-26. doi: 10.1016/0305-0491(91)90093-s.

Abstract

Elution profiles of 11 coral snake venoms, including those of Micrurus albicinctus, M. corallinus, M. frontalis altirostris, M. f. brasiliensis, M. f. frontalis, M. fulvius fulvius, M. ibiboboca, M. lemniscatus ssp., M. rondonianus, M. spixii spixii and M. surinamensis surinamensis, were compared using high performance gel filtration and reverse phase media. 2. Micrurus venom profiles were compared with those of "outgroup" taxa Bothrops moojeni, Naja naja kaouthia and Bungarus multicinctus. 3. Purified elapid venom constituents were also chromatographed under identical conditions in order to suggest possible identities of Micrurus venom constituents. 4. Masses of various components were confirmed by mass spectrometry. 5. Phospholipase constituents in three venoms were positively identified based on their reverse phase chromatograms. 6. Venoms of M. rondonianus and M. s. surinamensis are shown to be significantly different in their peptide composition from other Micrurus venoms.

摘要

使用高效凝胶过滤和反相介质比较了11种珊瑚蛇毒液的洗脱曲线，包括白环珊瑚蛇、珊瑚珊瑚蛇、高鼻珊瑚蛇指名亚种、巴西珊瑚蛇、珊瑚蛇指名亚种、佛罗里达珊瑚蛇指名亚种、伊比波博卡珊瑚蛇、带纹珊瑚蛇亚种、朗多尼亚珊瑚蛇、斯皮克斯珊瑚蛇指名亚种和苏里南珊瑚蛇的毒液洗脱曲线。2. 将珊瑚蛇毒液图谱与“外群”分类单元的图谱进行了比较，这些“外群”分类单元包括莫氏矛头蝮、眼镜王蛇和多带环蛇。3. 为了推测珊瑚蛇毒液成分的可能身份，还在相同条件下对纯化的眼镜蛇科毒液成分进行了色谱分析。4. 通过质谱法确认了各种成分的质量。5. 根据三种毒液的反相色谱图，阳性鉴定了其中的磷脂酶成分。6. 朗多尼亚珊瑚蛇和苏里南珊瑚蛇的毒液在肽组成上与其他珊瑚蛇毒液有显著差异。