珊瑚蛇蛇毒的电泳和免疫化学研究。

Electrophoretic and immunochemical studies of Micrurus snake venoms.

作者信息

Alape-Girón A, Lomonte B, Gustafsson B, Da Silva N J, Thelestam M

机构信息

Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San Jose.

出版信息

Toxicon. 1994 Jun;32(6):713-23. doi: 10.1016/0041-0101(94)90340-9.

DOI:10.1016/0041-0101(94)90340-9

PMID:7940577

Abstract

The electrophoretic mobilities of venom components from 15 Micrurus species were studied by polyacrylamide gel electrophoresis. The venoms showed species-specific protein patterns under native (PAGE) or denaturing (urea-PAGE) conditions. However, electrophoretic patterns obtained by SDS-PAGE under reducing conditions were similar. The proteins of all venoms had mol. wts either in the range of 45 to 75 kDa or lower than 14.5 kDa. PAGE and urea-PAGE of single extraction venom samples from 22 M. nigrocinctus nigrocinctus specimens revealed some proteins completely conserved, whereas others exhibited intraspecies variation. Based on ELISA cross-reactivity studies with 11 monoclonal antibodies against M. n. nigrocinctus venom, venoms from M. n. nigrocinctus, M. nigrocinctus mosquitensis, M. fulvius fulvius, M. dumerilii carnicauda and M. albicinctus were included in the same antigenic group, whereas M. frontalis frontalis and M. frontalis braziliensis venoms constituted a second group. Micrurus alleni and M. spixii spixii showed reactivity patterns similar to groups 1 and 2, respectively. Venoms from M. surinamensis surinamensis, M. corallinus, M. ibiboboca, M. hemiprichii ortoni, M. lemniscatus helleri and M. mipartitus had unique cross-reactivity patterns with monoclonal antibodies against M. n. nigrocinctus venom.

摘要

通过聚丙烯酰胺凝胶电泳研究了15种珊瑚蛇属物种毒液成分的电泳迁移率。在天然（PAGE）或变性（尿素-PAGE）条件下，毒液呈现出物种特异性的蛋白质图谱。然而，在还原条件下通过SDS-PAGE获得的电泳图谱相似。所有毒液的蛋白质分子量要么在45至75 kDa范围内，要么低于14.5 kDa。对22个黑带珊瑚蛇指名亚种标本的单次提取毒液样本进行PAGE和尿素-PAGE分析，结果显示一些蛋白质完全保守，而其他蛋白质则表现出种内变异。基于对11种抗黑带珊瑚蛇指名亚种毒液单克隆抗体的ELISA交叉反应性研究，黑带珊瑚蛇指名亚种、黑带珊瑚蛇蚊蛇亚种、佛罗里达珊瑚蛇指名亚种、杜氏珊瑚蛇卡尼卡达亚种和白纹珊瑚蛇的毒液属于同一抗原组，而中美珊瑚蛇指名亚种和中美珊瑚蛇巴西亚种的毒液构成第二组。艾伦氏珊瑚蛇和斯皮氏珊瑚蛇的反应模式分别与第1组和第2组相似。苏里南珊瑚蛇苏里南亚种、珊瑚珊瑚蛇、伊比波博卡珊瑚蛇、奥托尼半带珊瑚蛇、赫勒红带珊瑚蛇和米帕蒂图斯珊瑚蛇的毒液与抗黑带珊瑚蛇指名亚种毒液的单克隆抗体具有独特的交叉反应模式。