表皮、腭上皮和颊上皮中糖苷酶活性的比较。

Comparison of glycosidase activities in epidermis, palatal epithelium and buccal epithelium.

Chang F, Wertz P W, Squier C A

Dows Institute for Dental Research, University of Iowa, Iowa City 52242.

Comp Biochem Physiol B. 1991;100(1):137-9. doi: 10.1016/0305-0491(91)90096-v.

Abstract

beta-Glucosidase, alpha-glucosidase, beta-galactosidase and alpha-mannosidase were measured in epidermis, palatal and buccal epithelium of the pig (Sus scrofa). 2. All three epithelia contained similar alpha-mannosidase activity (1.7-3.2 nmol mg tissue-1 hr-1 at pH 4), and none contained significant alpha-glucosidase. 3. Specific activity of beta-glucosidase was high (9-13 nmol mg tissue-1 hr-1 at pH 4) in epidermis and palate, but activity was low (less than 2 nmol mg tissue-1 hr-1) in buccal epithelium. 4. Only epidermis contained a high level of beta-galactosidase (5.8 nmol mg tissue-1 hr-1). 5. Differences in glycosidase profiles may underlie differences in permeability barrier properties in these epithelia.

摘要

在猪（野猪）的表皮、腭和颊上皮中检测了β-葡萄糖苷酶、α-葡萄糖苷酶、β-半乳糖苷酶和α-甘露糖苷酶。2. 所有这三种上皮都含有相似的α-甘露糖苷酶活性（在pH 4时为1.7 - 3.2 nmol mg组织⁻¹小时⁻¹），且均不含显著的α-葡萄糖苷酶。3. β-葡萄糖苷酶的比活性在表皮和腭中较高（在pH 4时为9 - 13 nmol mg组织⁻¹小时⁻¹），但在颊上皮中活性较低（低于2 nmol mg组织⁻¹小时⁻¹）。4. 仅表皮含有高水平的β-半乳糖苷酶（5.8 nmol mg组织⁻¹小时⁻¹）。5. 糖苷酶谱的差异可能是这些上皮通透性屏障特性差异的基础。