钻石背响尾蛇和东部菱背响尾蛇毒液中小碱性肽毒素含量的区域差异。
Regional differences in content of small basic peptide toxins in the venoms of Crotalus adamanteus and Crotalus horridus.
作者信息
Straight R C, Glenn J L, Wolt T B, Wolfe M C
机构信息
Venom Research Laboratory, Veterans Affairs Medical Center, Salt Lake City, UT 84148.
出版信息
Comp Biochem Physiol B. 1991;100(1):51-8. doi: 10.1016/0305-0491(91)90083-p.
PMID:1756621
Abstract
- Reverse-phase HPLC and organic solvents were used to isolate small basic peptide (SBP) toxins from the venoms of Crotalus adamanteus, C. durissus terrificus, C. horridus, C. scutulatus scutulatus, C. viridis concolor, C. viridis helleri and C. viridis viridis. 2. Acid-DEP analyses indicated a high degree of toxin purity which was obtained with a single HPLC run. 3. The combined results of HPLC, immunodiffusion and electrophoresis analyses of venoms from different geographical regions indicate that the SBP toxin content in the venoms of Crotalus adamanteus, Crotalus horridus, Crotalus scutulatus and Crotalus viridis viridis may vary regionally.
摘要
- 采用反相高效液相色谱法（Reverse-phase HPLC）和有机溶剂从金刚王眼镜蛇（Crotalus adamanteus）、三色矛头蝮（C. durissus terrificus）、东部菱斑响尾蛇（C. horridus）、莫哈韦响尾蛇（C. scutulatus scutulatus）、草原响尾蛇（C. viridis concolor）、黑尾草原响尾蛇（C. viridis helleri）和西部草原响尾蛇（C. viridis viridis）的毒液中分离出小碱性肽（SBP）毒素。2. 酸性脱盐洗脱分析表明，单次高效液相色谱运行即可获得高度纯净的毒素。3. 对来自不同地理区域的毒液进行高效液相色谱、免疫扩散和电泳分析的综合结果表明，金刚王眼镜蛇、东部菱斑响尾蛇、莫哈韦响尾蛇和西部草原响尾蛇毒液中的SBP毒素含量可能因地区而异。
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