高效液相色谱分离的响尾蛇毒液的比较酶学研究。
Comparative enzymatic study of HPLC-fractionated Crotalus venoms.
作者信息
Soto J G, Perez J C, Lopez M M, Martinez M, Quintanilla-Hernandez T B, Santa-Hernandez M S, Turner K, Glenn J L, Straight R C, Minton S A
机构信息
Department of Biology, Texas A&I University, Kingsville 78363.
出版信息
Comp Biochem Physiol B. 1989;93(4):847-55. doi: 10.1016/0305-0491(89)90056-4.
PMID:2680253
Abstract
- Ten venoms of the genus Crotalus (Crotalus adamanteus, Crotalus atrox, Crotalus durissus durissus, Crotalus horridus horridus, Crotalus lepidus, Crotalus polystictus, Crotalus molossus molossus, Crotalus pusillus, Crotalus scutulatus scutulatus, venom B, and Crotalus viridis lutosus) were fractionated using HPLC anion and cation exchange chromatography. 2. HPLC venom fractions were tested for hemorrhagic, hemolytic, and proteolytic activities. 3. Crude Virginia opossum (Didelphis virginiana) serum neutralized the hemorrhagic activity of HPLC fractions.
摘要
- 使用高效液相色谱阴离子和阳离子交换色谱法对10种响尾蛇属毒液（东部菱斑响尾蛇、西部菱斑响尾蛇、杜氏响尾蛇、虎斑响尾蛇、细纹响尾蛇、多斑响尾蛇、墨西哥小响尾蛇、小响尾蛇、莫哈韦响尾蛇毒液B以及草原响尾蛇）进行了分离。2. 对高效液相色谱毒液组分进行了出血、溶血和蛋白水解活性测试。3. 弗吉尼亚负鼠的粗血清中和了高效液相色谱组分的出血活性。
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