Gu Congying, Lin Li, Chen Xiaodong, Jia Jinping, Ren Jicun, Fang Nenghu
College of Environmental Science & Engineering, Shanghai Jiaotong University, Shanghai, China.
J Sep Sci. 2007 May;30(7):1005-12. doi: 10.1002/jssc.200600397.
In this paper, a poly(styrene-octadecene-divinylbenzene) (PS-OD-DVB) monolithic column was prepared in one step by introducing a C18 carbon chain as monomer. N,N-Dimethylformamide and decanol served as porogens to make a homogeneous polymerization mixture in a fused silica capillary (320 microm inner diameter). Its physical and chromatographic properties were compared with those of poly(styrene-divinylbenzene) (PS-DVB) monolithic column, which was also fabricated by in-situ polymerization in a fused silica capillary with the same inner diameter. Six standard proteins were used to evaluate the columns and their potential application for the separation of human hemoglobin was also discussed. It was shown that the PS-OD-DVB and PS-DVB monoliths appeared to have similar efficiency for rapid separation of six proteins within 3.5 min. The PS-OD-DVB monolith was found to have higher loading capacity and higher resolution for the separation of alpha and beta chains of hemoglobin because of the introduction of C18 carbon chains, and shows great potential for the separation of bio-macromolecules.
在本文中，通过引入C18碳链作为单体一步法制备了聚（苯乙烯-十八烯-二乙烯基苯）（PS-OD-DVB）整体柱。N，N-二甲基甲酰胺和癸醇用作致孔剂，以在熔融石英毛细管（内径320微米）中形成均匀的聚合混合物。将其物理和色谱性能与同样通过原位聚合在相同内径的熔融石英毛细管中制备的聚（苯乙烯-二乙烯基苯）（PS-DVB）整体柱进行了比较。使用六种标准蛋白质对这些柱子进行评估，并讨论了它们在分离人血红蛋白方面的潜在应用。结果表明，PS-OD-DVB和PS-DVB整体柱在3.5分钟内对六种蛋白质进行快速分离时似乎具有相似的效率。由于引入了C18碳链，发现PS-OD-DVB整体柱在分离血红蛋白的α链和β链时具有更高的负载能力和更高的分辨率，并且在生物大分子的分离方面显示出巨大的潜力。
