Handa-Miya Satoko, Kimura Bon, Takahashi Hajime, Sato Miki, Ishikawa Tatsuya, Igarashi Kazunori, Fujii Tateo
Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Japan.
Int J Food Microbiol. 2007 Jul 15;117(3):312-8. doi: 10.1016/j.ijfoodmicro.2007.05.003. Epub 2007 May 17.
InlA is a surface protein participating in the entry of Listeria monocytogenes into mammalian non-phagocytic cells. PrfA is a positive regulatory factor that regulates the expression of a set of virulence genes. Recent studies revealed that some L. monocytogenes strains have a truncated form of these proteins because of nonsense mutations in their sequences, and these truncations contribute to the significant reduction in virulence of this pathogen. In this study, sequence analyses of inlA and prfA among L. monocytogenes isolated from ready-to-eat seafood revealed that only one out of 59 isolates had a nonsense-mutated inlA and all had non-mutated prfA. This indicated that these strains could be fully virulent based on the sizes of these proteins.
InlA是一种表面蛋白，参与单核细胞增生李斯特菌进入哺乳动物非吞噬细胞的过程。PrfA是一种正调控因子，可调节一组毒力基因的表达。最近的研究表明，一些单核细胞增生李斯特菌菌株由于其序列中的无义突变而具有这些蛋白的截短形式，这些截短导致该病原体的毒力显著降低。在本研究中，对从即食海鲜中分离出的单核细胞增生李斯特菌的inlA和prfA进行序列分析，结果显示59株分离株中只有1株inlA发生了无义突变，所有菌株的prfA均未发生突变。这表明基于这些蛋白的大小，这些菌株可能具有完全的毒力。
