Tomson Phillip L, Grover Liam M, Lumley Philip J, Sloan Alastair J, Smith Anthony J, Cooper Paul R
Oral Biology, School of Dentistry, The University of Birmingham, St Chad's Queensway, Birmingham B4 6NN, UK.
J Dent. 2007 Aug;35(8):636-42. doi: 10.1016/j.jdent.2007.04.008. Epub 2007 Jun 12.
To analyze the soluble components of setting and set mineral trioxide aggregate (MTA), assess the abilities of two varieties of MTA and Ca(OH)(2) solutions to solubilise dentine matrix proteins (DMPs) and determine if these extracts contain signalling molecules important to pulpal repair and regeneration.
The metallic ion composition of solutions of white and grey MTA (pH 11.7), 0.02M Ca(OH)(2) (pH 11.9) and 10% EDTA (pH 7.2) was determined using atomic absorption spectroscopy. Extracellular dentine matrix components from powdered human dentine were extracted using all solutions over 14 days. Extracts were analysed for concentrations of non-collagenous proteins (NCPs) and glycosaminoglycans (GAGs), and protein profiles were examined using 1D-polyacrylamide gel electrophoresis (1D-PAGE). ELISAs for TGF-beta1 and adrenomedullin (ADM) were also performed.
Aluminium, calcium, potassium, and sodium ions were detected in both white and grey MTA solutions. MTA and Ca(OH)(2) solutions liberated similar amounts of GAGs and NCPs although yields were considerably lower than those obtained using the EDTA solution. 1D-PAGE analysis demonstrated differences in protein profiles solubilised from dentine for all solutions. All extracts contained TGF-beta1 and ADM, EDTA solution liberated significantly greater amounts of TGF-beta1, and Ca(OH)(2) and grey MTA solutions released more ADM.
These data imply that when placed clinically soluble components of set and setting MTA may release dentine matrix components that potentially influence cellular events for dentine repair and regeneration.
分析凝固型和凝固后的矿物三氧化物凝聚体（MTA）的可溶性成分，评估两种MTA和氢氧化钙（Ca(OH)₂）溶液溶解牙本质基质蛋白（DMPs）的能力，并确定这些提取物是否含有对牙髓修复和再生重要的信号分子。
使用原子吸收光谱法测定白色和灰色MTA溶液（pH 11.7）、0.02M Ca(OH)₂溶液（pH 11.9）和10%乙二胺四乙酸（EDTA）溶液（pH 7.2）中的金属离子组成。在14天内使用所有溶液从粉末状人牙本质中提取细胞外牙本质基质成分。分析提取物中非胶原蛋白（NCPs）和糖胺聚糖（GAGs）的浓度，并使用一维聚丙烯酰胺凝胶电泳（1D-PAGE）检测蛋白质谱。还进行了转化生长因子-β1（TGF-β1）和肾上腺髓质素（ADM）的酶联免疫吸附测定（ELISA）。
在白色和灰色MTA溶液中均检测到铝、钙、钾和钠离子。MTA和Ca(OH)₂溶液释放的GAGs和NCPs量相似，尽管产量远低于使用EDTA溶液获得的产量。1D-PAGE分析表明，所有溶液从牙本质中溶解的蛋白质谱存在差异。所有提取物均含有TGF-β1和ADM，EDTA溶液释放的TGF-β1量显著更多，Ca(OH)₂和灰色MTA溶液释放的ADM更多。
这些数据表明，在临床应用时，凝固型和凝固后的MTA的可溶性成分可能释放牙本质基质成分，这些成分可能影响牙本质修复和再生的细胞事件。
