Li L-H, Wu L-J, Tashiro S-I, Onodera S, Uchiumi F, Ikejima T
China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang, China.
J Asian Nat Prod Res. 2007 Apr-Aug;9(3-5):245-52. doi: 10.1080/10286020600604260.
Silymarin, derived from the milk thistle plant, Silybum marianum, has been traditionally used in the treatment of liver disease. Our previous study demonstrated that silymarin has an anti-apoptotic effect against UV irradiation. In this study, SIRT1, a human deacetylase that was reported to promote cell survival, was activated by silymarin (5 x 10(- 4) mol/L) in UV-irradiated human malignant melanoma, A375-S2 cells, followed by down-regulated expression of Bax and decreased release of cytochrome c. Cleavage of procaspase-3 and digestion of its substrates, the inhibitor of caspase-activated DNase (ICAD) and poly(ADP-ribose) polymerase (PARP), were also reduced. Consistent with its protective effect on UV-induced apoptosis, silymarin (5 x 10(- 4) mol/L) also increased G(2)/M phase arrest, possibly providing a prolonged time for efficient DNA repair. Consequently, that silymarin protected A375-S2 cell against UV-induced apoptosis was partially through SIRT1 pathway and modulation of the cell cycle distribution.
水飞蓟素源自水飞蓟属植物水飞蓟，传统上用于治疗肝脏疾病。我们之前的研究表明，水飞蓟素对紫外线照射具有抗凋亡作用。在本研究中，据报道可促进细胞存活的人类去乙酰化酶SIRT1在紫外线照射的人类恶性黑色素瘤A375 - S2细胞中被水飞蓟素（5×10⁻⁴ mol/L）激活，随后Bax表达下调，细胞色素c释放减少。procaspase - 3的切割及其底物——半胱天冬酶激活的DNA酶抑制剂（ICAD）和聚（ADP - 核糖）聚合酶（PARP）的消化也减少。与其对紫外线诱导凋亡的保护作用一致，水飞蓟素（5×10⁻⁴ mol/L）还增加了G₂/M期阻滞，可能为有效的DNA修复提供了更长时间。因此，水飞蓟素保护A375 - S2细胞免受紫外线诱导凋亡部分是通过SIRT1途径和细胞周期分布的调节实现的。
