胶束和膜中膜蛋白的结构相似性。
Structural similarity of a membrane protein in micelles and membranes.
作者信息
Franzin Carla M, Teriete Peter, Marassi Francesca M
机构信息
Burnham Institute for Medical Research, La Jolla, CA 92037, USA.
出版信息
J Am Chem Soc. 2007 Jul 4;129(26):8078-9. doi: 10.1021/ja0728371. Epub 2007 Jun 13.
Abstract
The anisotropic spin interactions measured for membrane proteins in weakly oriented micelles and in oriented lipid bilayers provide independent and potentially complementary high-resolution restraints for structure determination. Here we show that the membrane protein CHIF adopts a similar structure in lipid micelles and bilayers, allowing the restraints from micelle and bilayer samples to be combined in a complementary fashion to enhance the structural information. Back-calculation and assignment of the NMR spectrum of CHIF in oriented lipid bilayers, from the structure determined in micelles, provides additional restraints for structure determination as well as the global orientation of the protein in the membrane. The combined use of solution and solid-state NMR restraints also affords cross-validation for the structural analysis.
摘要
在弱取向胶束和取向脂质双层中测量的膜蛋白各向异性自旋相互作用，为结构测定提供了独立且可能互补的高分辨率约束条件。在这里，我们表明膜蛋白CHIF在脂质胶束和双层中采用相似的结构，使得来自胶束和双层样品的约束条件能够以互补方式相结合，从而增强结构信息。根据在胶束中确定的结构，对取向脂质双层中CHIF的核磁共振谱进行反演和归属，为结构测定以及蛋白质在膜中的整体取向提供了额外的约束条件。溶液核磁共振和固态核磁共振约束条件的联合使用，也为结构分析提供了交叉验证。
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本文引用的文献
1
Structure of the Na,K-ATPase regulatory protein FXYD1 in micelles.胶束中钠钾ATP酶调节蛋白FXYD1的结构
Biochemistry. 2007 Jun 12;46(23):6774-83. doi: 10.1021/bi700391b. Epub 2007 May 19.
2
NMR of membrane proteins in micelles and bilayers: the FXYD family proteins.胶束和双层膜中膜蛋白的核磁共振：FXYD家族蛋白
Methods. 2007 Apr;41(4):398-408. doi: 10.1016/j.ymeth.2006.08.011.
3
PIPATH: an optimized algorithm for generating alpha-helical structures from PISEMA data.PIPATH：一种用于从PISEMA数据生成α-螺旋结构的优化算法。
J Magn Reson. 2006 Nov;183(1):87-95. doi: 10.1016/j.jmr.2006.07.020. Epub 2006 Aug 17.
4
Role of FXYD proteins in ion transport.FXYD蛋白在离子转运中的作用。
Annu Rev Physiol. 2006;68:431-59. doi: 10.1146/annurev.physiol.68.040104.131852.
5
Correlation of gene and protein structures in the FXYD family proteins.FXYD家族蛋白中基因与蛋白质结构的相关性
J Mol Biol. 2005 Dec 9;354(4):743-50. doi: 10.1016/j.jmb.2005.10.018. Epub 2005 Oct 28.
6
Structure determination of membrane proteins by NMR spectroscopy.利用核磁共振光谱法测定膜蛋白的结构
Chem Rev. 2004 Aug;104(8):3587-606. doi: 10.1021/cr0304121.
7
Residual dipolar couplings in structure determination of biomolecules.生物分子结构测定中的剩余偶极耦合
Chem Rev. 2004 Aug;104(8):3519-40. doi: 10.1021/cr030419i.
8
Simultaneous assignment and structure determination of a membrane protein from NMR orientational restraints.基于核磁共振取向限制同时确定膜蛋白的归属和结构
Protein Sci. 2003 Mar;12(3):403-11. doi: 10.1110/ps.0211503.
9
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Dipolar couplings in macromolecular structure determination.大分子结构测定中的偶极耦合
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