Franzin Carla M, Teriete Peter, Marassi Francesca M
Burnham Institute for Medical Research, La Jolla, CA 92037, USA.
Magn Reson Chem. 2007 Dec;45 Suppl 1(Suppl 1):S192-7. doi: 10.1002/mrc.2144.
Corticosteroid hormone-induced factor (CHIF) is a major regulatory subunit of the Na,K-ATPase, and a member of an evolutionarily conserved family of membrane proteins that regulate the function of the enzyme complex in a tissue-specific and physiological-state-specific manner. Here we present the structure of CHIF oriented in the membrane, determined by solid-state NMR orientation-dependent restraints. Because CHIF adopts a similar structure in lipid micelles and bilayers, it is possible to assign the solid-state NMR spectrum measured for (15)N-labeled CHIF in oriented bilayers from the structure determined in micelles, to obtain the global orientation of the protein in the membrane.
皮质类固醇激素诱导因子（CHIF）是钠钾ATP酶的主要调节亚基，属于一个在进化上保守的膜蛋白家族，该家族以组织特异性和生理状态特异性的方式调节酶复合物的功能。在此，我们展示了通过固态核磁共振取向依赖性限制确定的、定位于膜中的CHIF结构。由于CHIF在脂质微团和双层膜中采用相似结构，因此可以根据在微团中确定的结构，对在取向双层膜中测量的（15）N标记CHIF的固态核磁共振谱进行归属，以获得蛋白质在膜中的整体取向。
