Shanghai Institute of Ceramics, Chinese Academy of Science, Shanghai, People's Republic of China.
J R Soc Interface. 2013 May 1;10(84):20130070. doi: 10.1098/rsif.2013.0070. Print 2013 Jul 6.
Layer-by-layer (LBL) self-assembly technique has been proved to be a highly effective method to immobilize the main components of the extracellular matrix such as collagen and hyaluronic acid on titanium-based implants and form a polyelectrolyte multilayer (PEM) film by electrostatic interaction. However, the formed PEM film is unstable in the physiological environment and affects the long-time effectiveness of PEM film. In this study, a modified LBL technology has been developed to fabricate a stable collagen/hyaluronic acid (Col/HA) PEM film on titanium coating (TC) by introducing covalent immobilization. Scanning electron microscopy, diffuse reflectance Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy were used to characterize the PEM film. Results of Sirius red staining demonstrated that the chemical stability of PEM film was greatly improved by covalent cross-linking. Cell culture assays further illustrated that the functions of human mesenchymal stem cells, such as attachment, spreading, proliferation and differentiation, were obviously enhanced by the covalently immobilized Col/HA PEM on TCs compared with the absorbed Col/HA PEM. The improved stability and biological properties of the Col/HA PEM covalently immobilized TC may be beneficial to the early osseointegration of the implants.
层层自组装（LBL）技术已被证明是一种将细胞外基质的主要成分（如胶原蛋白和透明质酸）固定在钛基植入物上并通过静电相互作用形成聚电解质多层（PEM）膜的有效方法。然而，形成的 PEM 膜在生理环境中不稳定，影响 PEM 膜的长期效果。在这项研究中，通过引入共价固定化，开发了一种改良的 LBL 技术，在钛涂层（TC）上制备稳定的胶原蛋白/透明质酸（Col/HA）PEM 膜。扫描电子显微镜、漫反射傅里叶变换红外光谱和 X 射线光电子能谱用于表征 PEM 膜。天狼星红染色结果表明，通过共价交联，PEM 膜的化学稳定性得到了很大提高。细胞培养实验进一步表明，与吸收的 Col/HA PEM 相比，共价固定在 TC 上的 Col/HA PEM 明显增强了人骨髓间充质干细胞的黏附、铺展、增殖和分化等功能。共价固定 Col/HA PEM 的 TC 的稳定性和生物学性能得到改善，可能有利于植入物的早期骨整合。
