Allpike Bradley P, Heitz Anna, Joll Cynthia A, Kagi Robert I
Curtin Water Quality Research Centre, Centre for Applied Organic Geochemistry, Curtin University of Technology, G.P.O. Box U1987, Perth, WA 6845, Australia.
J Chromatogr A. 2007 Jul 20;1157(1-2):472-6. doi: 10.1016/j.chroma.2007.05.073. Epub 2007 May 29.
A novel organic carbon detector (OCD) for size exclusion chromatography (SEC), and its application to the characterisation of aquatic natural organic matter (NOM) in natural and treated potable water samples, is described. The instrument uses a conventional UV-persulfate oxidation technique to convert organic carbon to CO(2). The novelty of the technique is detection of the evolved CO(2) using a sensitive Fourier transform infrared (FTIR) spectroscopy 'lightpipe' detector originally designed for detection of analytes after gas chromatographic separation. With the exception of the lightpipe, the OCD system was constructed using simple, inexpensive, readily available components. The system was designed to minimise deadvolume, allowing for use of smaller sample sizes and smaller columns, substantially shortening analysis time, while maintaining chromatographic integrity through the OCD system. Downscaling resulted in some loss of separation but it was shown that this was caused by the lower separation efficiency of the smaller capacity column, rather than from sample dispersion within the OCD system.
描述了一种用于尺寸排阻色谱（SEC）的新型有机碳检测器（OCD）及其在天然和处理后的饮用水样品中水生天然有机物（NOM）表征中的应用。该仪器采用传统的紫外 - 过硫酸盐氧化技术将有机碳转化为CO₂。该技术的新颖之处在于使用最初设计用于气相色谱分离后分析物检测的灵敏傅里叶变换红外（FTIR）光谱“光管”检测器来检测逸出的CO₂。除了光管之外，OCD系统使用简单、廉价且易于获得的组件构建。该系统旨在将死体积降至最低，允许使用更小的样品量和更小的色谱柱，从而大幅缩短分析时间，同时通过OCD系统保持色谱完整性。缩小规模导致了一些分离度损失，但结果表明这是由较小容量色谱柱的较低分离效率引起的，而非OCD系统内的样品分散所致。
