Simões Mariana, Bahia Diana, Zerlotini Adhemar, Torres Kleider, Artiguenave François, Neshich Goran, Kuser Paula, Oliveira Guilherme
Laboratory of Cellular and Molecular Parasitology, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Av. Augusto de Lima 1715, Belo Horizonte 30190-002, MG, Brazil.
Mol Biochem Parasitol. 2007 Aug;154(2):134-40. doi: 10.1016/j.molbiopara.2007.04.003. Epub 2007 Apr 13.
Single nucleotide polymorphism (SNP) markers have been shown to be useful in genetic investigations of medically important parasites and their hosts. In this paper, we describe the prediction and validation of SNPs in ESTs of Schistosoma mansoni. We used 107,417 public sequences of S. mansoni and identified 15,614 high-quality candidate SNPs in 12,184 contigs. The presence of predicted SNPs was observed in well characterized antigens and vaccine candidates such as those coding for myosin; Sm14 and Sm23; cathepsin B and triosephosphate isomerase (TPI). Additionally, SNPs were experimentally validated for the cathepsin B. A comparative model of the S. mansoni cathepsin B was built for predicting the possible consequences of amino acid substitutions on the protein structure. An analysis of the substitutions indicated that the amino acids were mostly located on the surface of the molecule, and we found no evidence for a significant conformational change of the enzyme. However, at least one of the substitutions could result in a structural modification of an epitope.
单核苷酸多态性（SNP）标记已被证明在医学上重要的寄生虫及其宿主的遗传研究中很有用。在本文中，我们描述了曼氏血吸虫ESTs中SNP的预测和验证。我们使用了107,417条曼氏血吸虫的公共序列，并在12,184个重叠群中鉴定出15,614个高质量的候选SNP。在特征明确的抗原和候选疫苗中观察到预测SNP的存在，例如那些编码肌球蛋白、Sm14和Sm23、组织蛋白酶B和磷酸丙糖异构酶（TPI）的抗原。此外，对组织蛋白酶B的SNP进行了实验验证。构建了曼氏血吸虫组织蛋白酶B的比较模型，以预测氨基酸取代对蛋白质结构的可能影响。对取代的分析表明，氨基酸大多位于分子表面，并且我们没有发现该酶发生显著构象变化的证据。然而，至少有一个取代可能导致表位的结构修饰。
