dos Reis M G, Davis R E, Singh H, Skelly P J, Shoemaker C B
Harvard School of Public Health, Department of Tropical Public Health, Boston, MA 02115.
Mol Biochem Parasitol. 1993 Jun;59(2):235-42. doi: 10.1016/0166-6851(93)90221-i.
The complete gene encoding Schistosoma mansoni triosephosphate isomerase (TPI) was isolated from a lambda phage genomic library on 2 overlapping clones. These genomic clones have been characterized by restriction mapping and DNA sequencing of the 5' flanking region, the exons, the intron boundaries and the polyadenylation addition site. S. mansoni TPI is encoded by 6 exons spanning a region of about 12 kb. The 5 introns are located at positions precisely analogous to those of mammalian TPI genes but one of the 6 mammalian TPI introns is missing in S. mansoni. We find no evidence of spliced leader involvement in TPI gene expression. The gene is preceded by at least 4 tandem copies of a 2.5-kb repetitive sequence. While the 12-kb size for the S. mansoni TPI gene is much larger than the 3-4 kb typical of mammalian TPI genes, the 42-bp first intron is unusually short. The transcription initiation site for the S. mansoni TPI gene is heterogeneous. Genomic Southern blot analysis suggests that TPI is expressed from a single copy gene.
从一个λ噬菌体基因组文库的两个重叠克隆中分离出了编码曼氏血吸虫磷酸丙糖异构酶（TPI）的完整基因。这些基因组克隆已通过对5'侧翼区域、外显子、内含子边界和聚腺苷酸化加尾位点的限制性图谱分析和DNA测序进行了表征。曼氏血吸虫TPI由6个外显子编码，跨越约12 kb的区域。5个内含子位于与哺乳动物TPI基因的位置精确对应的位置，但曼氏血吸虫中缺少6个哺乳动物TPI内含子中的一个。我们没有发现剪接前导序列参与TPI基因表达的证据。该基因之前至少有4个2.5 kb重复序列的串联拷贝。虽然曼氏血吸虫TPI基因12 kb的大小比哺乳动物TPI基因典型的3 - 4 kb大得多，但42 bp的第一个内含子异常短。曼氏血吸虫TPI基因的转录起始位点是异质的。基因组Southern印迹分析表明TPI由单拷贝基因表达。
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