中国血浆捐献者中丙型肝炎病毒核心抗原检测用于丙型肝炎病毒感染的早期诊断
Detection of hepatitis C virus core antigen for early diagnosis of hepatitis C virus infection in plasma donor in China.
作者信息
Zhang He-Qiu, Li Shao-Bo, Wang Guo-Hua, Chen Kun, Song Xiao-Guo, Feng Xiao-Yan
机构信息
Department of Vaccine Engineering, Beijing Institute of Basic Medical Sciences, 27 Taiping Road, Haidian District, Beijing 100850, China.
出版信息
World J Gastroenterol. 2007 May 21;13(19):2738-42. doi: 10.3748/wjg.v13.i19.2738.
AIM
To evaluate the efficacy of a new hepatitis C virus (HCV) core antigen assay developed in China.
METHODS
After the determination of HCV infection, 49 serial samples were selected from 11 regular plasma donors in 5 different plasma stations. To compare the performance of HCV core antigen detection and HCV PCR, these samples were genotyped, and each specimen was analyzed by ELISA for the detection of HCV core antigen and by qualitative HCV PCR.
RESULTS
Among all of the sequential samples, the original 13 specimens were HCV RNA-negative, and 36 samples were HCV RNA-positive. Twenty-seven samples (75%) were HCV core antigen-positive from these HCV RNA-positive specimens. Conversely, 27 samples (93.1%) were found HCV RNA-positive in HCV core antigen-positive samples. Intervals between HCV RNA and HCV core antigen-positive, as well as between HCV core antigen-positive and HCV antibody-positive were 36.0 and 32.8 d, respectively.
CONCLUSION
This HCV core antigen assay, developed in China, is able to detect much of anti-HCV-negative, HCV RNA-positive preseroconversion window period (PWP) plasma donations.
目的
评估中国研发的一种新型丙型肝炎病毒（HCV）核心抗原检测方法的效能。
方法
确定HCV感染后，从5个不同血浆站的11名常规血浆捐献者中选取49份连续样本。为比较HCV核心抗原检测和HCV PCR的性能，对这些样本进行基因分型，每个样本通过ELISA检测HCV核心抗原，并进行定性HCV PCR分析。
结果
在所有连续样本中，最初的13份样本HCV RNA阴性，36份样本HCV RNA阳性。这些HCV RNA阳性样本中有27份（75%）HCV核心抗原阳性。相反，在HCV核心抗原阳性样本中，27份样本（93.1%）被检测出HCV RNA阳性。HCV RNA阳性与HCV核心抗原阳性之间以及HCV核心抗原阳性与HCV抗体阳性之间的间隔分别为36.0天和32.8天。
结论
中国研发的这种HCV核心抗原检测方法能够检测出许多抗-HCV阴性、HCV RNA阳性的血清转化前期（PWP）血浆捐献。
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