Güldür Tayfun, Bayraktar Nihayet, Kaynar Ozgür, Beker Gülçin, Koçer Muzaffer, Ozcan Hamdi
Department of Medical Biochemistry, Faculty of Medicine, Inönü University, Malatya, Turkey.
J Basic Clin Physiol Pharmacol. 2007;18(1):21-35. doi: 10.1515/jbcpp.2007.18.1.21.
Most of the lipids of the skin surface come from sebaceous glands secretions, called sebum. Some of the sebum lipids are synthesized by sebaceous cells while some are reportedly derived from the plasma. Role of blood lipoproteins in sebum secretion rate and composition is unclear. To this end, excretion rate and composition of skin surface lipids of normo- and type IV hyperlipoproteinemic subjects were compared.
Quantitative analysis of skin surface lipids was performed by three successive sampling on left, middle, and right zones of the forehead with a sebumeter. Skin surface lipid samples for the compositional analysis were collected from the forehead, extracted into n-hexane, and analyzed by high performance thin layer chromatography (HPTLC). HPTLC plates were scanned with a densitometer for the quantification of the lipids.
Skin surface lipids from type IV hyperlipoproteinemic subjects contained higher proportion of wax ester + cholesterol ester compared with that from normolipoproteinemic subjects. However, skin surface lipid excretion rates of normo- and hyperlipoproteinemic subjects were found to be similar.
Plasma lipid/lipoprotein concentration may be a determinant factor in sebum lipid composition.
皮肤表面的大多数脂质来自皮脂腺分泌物，即皮脂。一些皮脂脂质由皮脂腺细胞合成，而据报道一些则源自血浆。血脂蛋白在皮脂分泌率和成分中的作用尚不清楚。为此，比较了正常血脂和IV型高脂蛋白血症受试者皮肤表面脂质的排泄率和成分。
使用皮脂测定仪在前额的左、中、右区域连续三次采样，对皮肤表面脂质进行定量分析。用于成分分析的皮肤表面脂质样本从前额收集，用正己烷提取，并通过高效薄层色谱法（HPTLC）进行分析。用密度计扫描HPTLC板以定量脂质。
与正常血脂受试者相比，IV型高脂蛋白血症受试者的皮肤表面脂质中蜡酯+胆固醇酯的比例更高。然而，正常血脂和高脂蛋白血症受试者的皮肤表面脂质排泄率相似。
血浆脂质/脂蛋白浓度可能是皮脂脂质成分的决定因素。
