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切除修复不能保护枯草芽孢杆菌细胞免受阳光照射而失活。

[Excision repair does not protect Bacillus subtilis cells from inactivation by sunlight].

作者信息

Lotareva O V, Filippov V D

出版信息

Genetika. 1991 Jul;27(7):1264-8.

PMID:1756960
Abstract

Exponentially growing Bacillus subtilis cells are highly sensitive to inactivating action of sunlight, the strain deficient in excision repair being every more sensitive than the uvr1 mutant. The inactivating effect is connected with the action of irradiation located in the left part of the spectrum (the whole UV region and some zones of the visible one). Increased sensitivity to sunlight disappeared when cells were exposed to sunlight in a liquid medium with casaminoacids (2 g/l). The inactivating effect was probably of photodynamic nature and was caused either by DNA lesions that were not removed by excision repair or by the damage which arose not in DNA.

摘要

指数生长期的枯草芽孢杆菌细胞对阳光的灭活作用高度敏感,缺乏切除修复的菌株比uvr1突变体更敏感。这种灭活作用与光谱左侧部分(整个紫外线区域和可见光的一些区域)的辐射作用有关。当细胞在含有酪蛋白氨基酸(2 g/l)的液体培养基中暴露于阳光下时,对阳光的敏感性增加消失。这种灭活作用可能具有光动力性质,要么是由切除修复未去除的DNA损伤引起的,要么是由非DNA产生的损伤引起的。

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