用于检测感染山羊眼结膜支原体的TaqMan实时荧光定量PCR的验证及诊断效能
Validation and diagnostic efficacy of a TaqMan real-time PCR for the detection of Mycoplasma conjunctivae in the eyes of infected Caprinae.
作者信息
Vilei Edy M, Bonvin-Klotz Laetitia, Zimmermann Liza, Ryser-Degiorgis Marie-Pierre, Giacometti Marco, Frey Joachim
机构信息
Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
出版信息
J Microbiol Methods. 2007 Aug;70(2):384-6. doi: 10.1016/j.mimet.2007.05.007. Epub 2007 May 21.
Infectious keratoconjunctivitis (IKC), caused by Mycoplasma conjunctivae, is a highly contagious ocular disease in Caprinae. To detect rapidly and sensitively M. conjunctivae from individual conjunctival swabs of infected domestic and wild animals, a specific real-time PCR was developed using an lppS-directed hydrolysis probe in a TaqMan platform.
由结膜支原体引起的传染性角膜结膜炎（IKC）是一种在山羊中具有高度传染性的眼部疾病。为了从受感染的家畜和野生动物的单个结膜拭子中快速灵敏地检测结膜支原体，在TaqMan平台上使用基于lppS的水解探针开发了一种特异性实时PCR。
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